BioProject
KAP241433
BioProject Title
Formyl-methionine-mediated eukaryotic ribosome quality control pathway for cold adaptation
(DDBJ: PRJDB37362)
Common Fields
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BioProject
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KAP241433 |
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Taxonomic type
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Organism
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Saccharomyces cerevisiae
(4932)
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Biomaterial provider
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Prof. Cheol-Sang Hwang from Korea University
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Submission date
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2025-02-04 |
Accession
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Experiments
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BioSample [Model organism or animal]
Showing of 1-20 of 24 cases
| BioSample ID | Identifier | Sample name | Age | Biomaterial provider | Birth date | Birth location | Breed | Breeding history | Breeding method | Cell line | Cell subtype | Cell type | Collected by | Collection Date | Cultivar | Culture collection | Death Date | Derived from | Description | Development stage | Disease | Disease stage | Ecotype | Genotype | Geographic location | Growth protocol | Health state | Isolate | Isolation source | Latitude and longitude | NCBI taxonomy ID | Organism | Phenotype | Sample type | Sex | Specimen voucher | Storage conditions | Strain | Stud book number | Tissue | Treatment | Other characteristics | Registration Date |
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| KAS24109241 | DRA : SAMD01619482 | RLI1_depl R42L replicate 2 | Prof. Cheol-Sang Hwang from Korea University | RLI1_depl, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (RLI1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (RLI1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109240 | DRA : SAMD01619481 | RLI1_depl R42L replicate 1 | Prof. Cheol-Sang Hwang from Korea University | RLI1_depl, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (RLI1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (RLI1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109239 | DRA : SAMD01619480 | RLI1_depl EcFMT replicate 2 | Prof. Cheol-Sang Hwang from Korea University | RLI1_depl, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (RLI1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (RLI1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109238 | DRA : SAMD01619479 | RLI1_depl EcFMT replicate 1 | Prof. Cheol-Sang Hwang from Korea University | RLI1_depl, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (RLI1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (RLI1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109237 | DRA : SAMD01619478 | NIP1_depl R42L replicate 2 | Prof. Cheol-Sang Hwang from Korea University | NIP1_depl, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (NIP1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (NIP1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109236 | DRA : SAMD01619477 | NIP1_depl R42L replicate 1 | Prof. Cheol-Sang Hwang from Korea University | NIP1_depl, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (NIP1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (NIP1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109235 | DRA : SAMD01619476 | NIP1_depl EcFMT replicate 2 | Prof. Cheol-Sang Hwang from Korea University | NIP1_depl, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (NIP1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (NIP1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109234 | DRA : SAMD01619475 | NIP1_depl EcFMT replicate 1 | Prof. Cheol-Sang Hwang from Korea University | NIP1_depl, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (NIP1_depl) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (NIP1_depl) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109233 | DRA : SAMD01619474 | hcr1D R42L replicate 2 | Prof. Cheol-Sang Hwang from Korea University | hcr1D, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (hcr1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (hcr1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109232 | DRA : SAMD01619473 | hcr1D R42L replicate 1 | Prof. Cheol-Sang Hwang from Korea University | hcr1D, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (hcr1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (hcr1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109231 | DRA : SAMD01619472 | hcr1D EcFMT replicate 2 | Prof. Cheol-Sang Hwang from Korea University | hcr1D, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (hcr1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (hcr1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109230 | DRA : SAMD01619471 | hcr1D EcFMT replicate 1 | Prof. Cheol-Sang Hwang from Korea University | hcr1D, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (hcr1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (hcr1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109229 | DRA : SAMD01619470 | arf1D R42L replicate 2 | Prof. Cheol-Sang Hwang from Korea University | arf1D, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (arf1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (arf1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109228 | DRA : SAMD01619469 | arf1D R42L replicate 1 | Prof. Cheol-Sang Hwang from Korea University | arf1D, R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L (arf1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L (arf1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109227 | DRA : SAMD01619468 | arf1D EcFMT replicate 2 | Prof. Cheol-Sang Hwang from Korea University | arf1D, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (arf1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (arf1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109226 | DRA : SAMD01619467 | arf1D EcFMT replicate 1 | Prof. Cheol-Sang Hwang from Korea University | arf1D, EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT (arf1D) | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT (arf1D) | 2025-02-03 | |||||||||||||||||||||||
| KAS24109225 | DRA : SAMD01619466 | RNA MTFi replicate 2 | Prof. Cheol-Sang Hwang from Korea University | R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L | 2025-02-03 | |||||||||||||||||||||||
| KAS24109224 | DRA : SAMD01619465 | RNA MTFi replicate 1 | Prof. Cheol-Sang Hwang from Korea University | R42L | Not applicable | Not applicable | Not applicable | Not applicable | inactive EcFMT R42L | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | CHY909 | whole cell | inactive EcFMT R42L | 2025-02-03 | |||||||||||||||||||||||
| KAS24109223 | DRA : SAMD01619464 | RNA MTF replicate 2 | Prof. Cheol-Sang Hwang from Korea University | EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT | 2025-02-03 | |||||||||||||||||||||||
| KAS24109222 | DRA : SAMD01619463 | RNA MTF replicate 1 | Prof. Cheol-Sang Hwang from Korea University | EcFMT | Not applicable | Not applicable | Not applicable | Not applicable | Wild-type EcFMT | South Korea: Seoul | cells were grown to A600 of ~1 at 30 Degree C in 3ml of SC(-Leu) medium. Each culture (1 ml of cell suspension at A600 of 1) was washed twice in 1ml of distilled water, serially diluted 5-fold in 200 ml of distilled water, and then 5 ml of each cell suspension was spotted onto SC(-Ura) or SGly(-Ura) plates containing 2 percent fermentable glucose or 2 percent respirable glycerol, respectively. These plates were then incubated at 30 Degree C for 2 or 4 days. | Not applicable | 4932 | Saccharomyces cerevisiae | not applicable | Not applicable | W303-1A | whole cell | Wild-type EcFMT | 2025-02-03 |