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단백체 데이터

Accession

KPX10000277
Submission date

2026-08-07
Metadata export

XML Download

Project Detail

Dataset detail - Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol

BioProject

KAP242491

ProjectTitle

Time-Dependent Multi-Omics Integration Maps Ferroptosis as an Early Driver of Sepsis-Associated Acute Kidney Injury

Description

Time-dependent multi-omics integration (TDMI) is a strategy that enables the effective exploration of the mechanisms underlying rapidly progressing diseases through single-omics analyses based on the central dogma. Sepsis-associated acute kidney injury (SA-AKI) is a rapid progressive, severe, and life-threatening complication of sepsis; however, its early-stage molecular pathogenesis remains insufficiently characterized. In this study, an in vivo multi omics strategy with multiple time points was employed to elucidate the toxic mechanisms underlying SA-AKI. After cecal ligation and puncture (CLP), samples were collected for phosphoproteomic (4 h post-CLP), transcriptomic (8 h), proteomic (12 h), and metabolomic/lipidomic (24 h) analyses to sequentially capture the molecular events during sepsis progression. Differentially expressed molecules from each omics layer were then integrated using xMWAS and Reactome to enable network-level characterization of the underlying mechanisms. Integrative TDMI analysis implicated ferroptosis as a key mechanism contributing to SA-AKI progression. The involvement of ferroptosis was further supported by both in vitro and in vivo validation experiments using ferrostatin-1, a pharmacological inhibitor of ferroptosis. Collectively, these findings demonstrate that TDMI can provide mechanistic insights into the dynamic molecular interactions underlying SA-AKI and highlight ferroptosis as a potential therapeutic target in this multifactorial disease process.

Keywords

Submitter

Lee Sang kyu , Sungkyunkwan University

Publication

Publication
PubMed ID	DOI

Dataset Detail

Dataset detail - Description, Keywords, Principal investigato, Pubmed ID, Doi, Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol

Dataset Title

Sample 1

Submission Type

Species

Mus musculus (Mouse)

Sample type

Sample type
Body fluid	Tissue	Cell	Others
	kidney

Disease

Disease
Others - sepsis

Fractionation

Fractionation
Method	Separation mode	Number of fractions
Not applicable
Not applicable

Digestion

Trypsin, Trypsin

Quantification

Quantification
Labeling	Labeling Child	Plex
Isobaric labeling(TMT)	TMT
Isobaric labeling(18O)	18O

Modifications

Carbamidomethyl (C)

Modifications

Bottom-up proteomics, Bottom-up proteomics

MS instrument

Thermo Scientific Q Exactive HF-X, Thermo Scientific Q Exactive HF-X

Sample processing protocol

Data analysis protocol

Search parameters

searchParameter

Search engine

searchEngine
Software	Version
MaxQuant

FASTA DB

FASTA DB
Type	Version
Swiss-prot(Reviewed)

FASTA DB Species

Enzyme

Missed cleavages

Number of enzymatic termini

Precursor mass tolerance

Number of 13C

Number of 13C
Min	Max

Fragment mass tolerance

Fixed modifications

Others,

Variable modifications

Others,

Quantification method

FDR

FDR
Protein	PSM	Peptide

Supplementary information

Announce Date

2026-07-31

Files Summary

Total 106 files 115,645,046,112 115,645,046,112

Files Summary
File type	# Files	Total Size
raw	96	115,626,382,374 115,626,382,374
searchResultFile	10	18,663,738 18,663,738

file 목록
file 선택	File Name	Size	Type	Published
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1--4.raw	1,939,971,225 1,939,971,225	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1--6.raw	1,859,044,660 1,859,044,660	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1--8.raw	1,889,868,845 1,889,868,845	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1-1.raw	2,222,634,227 2,222,634,227	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1-2.raw	2,210,371,355 2,210,371,355	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1-3.raw	2,075,746,536 2,075,746,536	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1-5.raw	1,896,459,045 1,896,459,045	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-1-7.raw	2,010,236,170 2,010,236,170	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2--2.raw	2,157,992,633 2,157,992,633	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2--3.raw	1,982,365,202 1,982,365,202	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2-1.raw	2,104,302,936 2,104,302,936	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2-4.raw	1,855,874,765 1,855,874,765	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2-5.raw	1,902,921,741 1,902,921,741	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2-6.raw	1,872,982,598 1,872,982,598	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2-7.raw	1,978,805,376 1,978,805,376	raw	2026-07-31
file 선택	20230117_S-AKI_TMT_500ng_2hr_F1-2-8.raw	1,839,238,022 1,839,238,022	raw	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-1.mzML	1,010,751,516 1,010,751,516	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-1.mzML	1,010,751,516 1,010,751,516	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-1.mzML	1,010,751,516 1,010,751,516	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-1.mzML	1,010,751,516 1,010,751,516	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-1.mzML	1,010,751,516 1,010,751,516	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-1.raw	1,383,916,662 1,383,916,662	raw	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-2.mzML	1,042,848,686 1,042,848,686	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-2.mzML	1,042,848,686 1,042,848,686	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-2.mzML	1,042,848,686 1,042,848,686	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-2.mzML	1,042,848,686 1,042,848,686	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-2.mzML	1,042,848,686 1,042,848,686	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set1-2.raw	1,420,016,662 1,420,016,662	raw	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-1.mzML	1,028,762,280 1,028,762,280	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-1.mzML	1,028,762,280 1,028,762,280	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-1.mzML	1,028,762,280 1,028,762,280	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-1.mzML	1,028,762,280 1,028,762,280	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-1.mzML	1,028,762,280 1,028,762,280	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-1.raw	1,393,878,833 1,393,878,833	raw	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-2.mzML	1,063,637,770 1,063,637,770	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-2.mzML	1,063,637,770 1,063,637,770	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-2.mzML	1,063,637,770 1,063,637,770	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-2.mzML	1,063,637,770 1,063,637,770	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-2.mzML	1,063,637,770 1,063,637,770	mzML	2026-07-31
file 선택	20230207_S-AKI_18O_TiO2_new_set2-2.raw	1,422,723,531 1,422,723,531	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-1.raw	530,505,297 530,505,297	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-2.raw	553,230,083 553,230,083	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-3.raw	541,610,392 541,610,392	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-4.raw	553,033,229 553,033,229	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-5.raw	521,592,496 521,592,496	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-6.raw	528,492,242 528,492,242	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-7.raw	536,244,248 536,244,248	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(1)-8.raw	584,131,257 584,131,257	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-1.raw	501,241,248 501,241,248	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-2.raw	540,350,773 540,350,773	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-3.raw	528,048,060 528,048,060	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-4.raw	538,582,469 538,582,469	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-5.raw	518,721,870 518,721,870	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-6.raw	525,979,050 525,979,050	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-7.raw	545,404,489 545,404,489	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F1(2)-8.raw	591,831,459 591,831,459	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-1.raw	531,010,196 531,010,196	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-2.raw	552,650,873 552,650,873	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-3.raw	533,316,660 533,316,660	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-4.raw	535,810,905 535,810,905	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-5.raw	517,847,014 517,847,014	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-6.raw	539,722,073 539,722,073	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-7.raw	556,983,109 556,983,109	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(1)-8.raw	587,615,151 587,615,151	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-1.raw	537,051,381 537,051,381	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-2.raw	552,896,256 552,896,256	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-3.raw	546,510,987 546,510,987	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-4.raw	544,878,507 544,878,507	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-5.raw	523,511,966 523,511,966	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-6.raw	545,620,007 545,620,007	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-7.raw	553,273,973 553,273,973	raw	2026-07-31
file 선택	20230210_S-AKI_18O_TiO2_new_Global_F2(2)-8.raw	588,516,530 588,516,530	raw	2026-07-31
file 선택	Phospho (STY)Sites_S-AKI_new_V8_Final.xlsx	709,018 709,018	xlsx	2026-07-31
file 선택	Phospho (STY)Sites_S-AKI_new_V8_Final.xlsx	709,018 709,018	xlsx	2026-07-31
file 선택	Phospho (STY)Sites_S-AKI_new_V8_Final.xlsx	709,018 709,018	xlsx	2026-07-31
file 선택	Phospho (STY)Sites_S-AKI_new_V8_Final.xlsx	709,018 709,018	xlsx	2026-07-31
file 선택	Phospho (STY)Sites_S-AKI_new_V8_Final.xlsx	709,018 709,018	xlsx	2026-07-31
file 선택	Phospho (STY)Sites_S-AKI_new_V8_Final.xlsx	709,018 709,018	xlsx	2026-07-31
file 선택	TMT_proteinGroups_V10_normalization_Final.xlsx	6,495,797 6,495,797	xlsx	2026-07-31

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