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KoBMAP_Mouse Heart Proteomics workflow comparison_Phospho_DIA
  • Accession
    KPX10000260
  • Submission date
    2025-12-05
  • Metadata export
    XML Download
Project Detail
Dataset detail - Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol
BioProject
 KAP241926
ProjectTitle
 Mouse heart proteomics workflow comparison for KoBMAP project
Description
 To establish optimal analytical conditions for high-quality data generation, mouse heart tissue was used to evaluate different combinations of sample preparation and acquisition strategies. For global proteomics, In-solution, FASP, and S-trap digestion methods were assessed in combination with both DDA and DIA acquisition modes. For phosphoproteomics, TiO₂ and IMAC enrichment methods were evaluated together with DDA and DIA acquisition strategies.
Keywords
  
Submitter
 Kim Min-Sik , DGIST
Publication
Publication
PubMed ID DOI
   
Dataset Detail
Dataset detail - Description, Keywords, Principal investigato, Pubmed ID, Doi, Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol
Dataset Title
 KoBMAP_Mouse Heart Proteomics workflow comparison_Phospho_DIA
Submission Type
 Complete
Species
 Mus musculus (Mouse)
Sample type
Sample type
Body fluid Tissue Cell Others
heart
Disease
Disease
Fractionation
Fractionation
Method Separation mode Number of fractions
Digestion
 Trypsin
Quantification
Quantification
Labeling Labeling Child Plex
()
Modifications
Carbamidomethyl (C), Phospho (S), Phospho (T), Phospho (Y)
Modifications
Bottom-up proteomics
MS instrument
Thermo Scientific Orbitrap Exploris 480
Sample processing protocol
Data analysis protocol
Search parameters
Supplementary information
 
Announce Date
 2025-12-03
Files Summary

Total 19 files 3,312,667,877 3,312,667,877

Files Summary
File type # Files Total Size
raw 6 3,128,691,109 3,128,691,109
searchResultFile 13 183,976,768 183,976,768
File
file 목록
File Name Size Type Published Download mzMl QC file
Mouse_heart_IMAC_DIA_1.raw 518,290,219 518,290,219 raw 2025-12-03
Mouse_heart_IMAC_DIA_2.raw 546,083,945 546,083,945 raw 2025-12-03
Mouse_heart_IMAC_DIA_3.raw 516,577,454 516,577,454 raw 2025-12-03
Mouse_heart_TiO2_DIA_1.raw 514,826,791 514,826,791 raw 2025-12-03
Mouse_heart_TiO2_DIA_2.raw 518,360,652 518,360,652 raw 2025-12-03
Mouse_heart_TiO2_DIA_3.raw 514,552,048 514,552,048 raw 2025-12-03
report-first-pass.gg_matrix.tsv 161,681 161,681 tsv 2025-12-03
report-first-pass.pg_matrix.tsv 380,942 380,942 tsv 2025-12-03
report-first-pass.pr_matrix.tsv 2,908,965 2,908,965 tsv 2025-12-03
report-first-pass.stats.tsv 1,496 1,496 tsv 2025-12-03
report-first-pass.tsv 46,055,689 46,055,689 tsv 2025-12-03
report-first-pass.unique_genes_matrix.tsv 157,611 157,611 tsv 2025-12-03
report-lib.tsv 81,694,944 81,694,944 tsv 2025-12-03
report.gg_matrix.tsv 169,346 169,346 tsv 2025-12-03
report.pg_matrix.tsv 388,074 388,074 tsv 2025-12-03
report.pr_matrix.tsv 2,866,274 2,866,274 tsv 2025-12-03
report.stats.tsv 1,506 1,506 tsv 2025-12-03
report.tsv 49,024,999 49,024,999 tsv 2025-12-03
report.unique_genes_matrix.tsv 165,241 165,241 tsv 2025-12-03