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KoBMAP_Mouse Heart Proteomics workflow comparison_Phospho_DDA
  • Accession
    KPX10000259
  • Submission date
    2025-12-03
  • Metadata export
    XML Download
Project Detail
Dataset detail - Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol
BioProject
 KAP241926
ProjectTitle
 Mouse heart proteomics workflow comparison for KoBMAP project
Description
 To establish optimal analytical conditions for high-quality data generation, mouse heart tissue was used to evaluate different combinations of sample preparation and acquisition strategies. For global proteomics, In-solution, FASP, and S-trap digestion methods were assessed in combination with both DDA and DIA acquisition modes. For phosphoproteomics, TiO₂ and IMAC enrichment methods were evaluated together with DDA and DIA acquisition strategies.
Keywords
  
Submitter
 Kim Min-Sik , DGIST
Publication
Publication
PubMed ID DOI
   
Dataset Detail
Dataset detail - Description, Keywords, Principal investigato, Pubmed ID, Doi, Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol
Dataset Title
 KoBMAP_Mouse Heart Proteomics workflow comparison_Phospho_DDA
Submission Type
 Complete
Species
 Mus musculus (Mouse)
Sample type
Sample type
Body fluid Tissue Cell Others
heart
Disease
Disease
Fractionation
Fractionation
Method Separation mode Number of fractions
Digestion
Quantification
Quantification
Labeling Labeling Child Plex
()
Modifications
Carbamidomethyl (C), Phospho (S), Phospho (T), Phospho (Y)
Modifications
Bottom-up proteomics
MS instrument
Thermo Scientific Orbitrap Exploris 480
Sample processing protocol
Data analysis protocol
Search parameters
Supplementary information
 
Announce Date
 2025-12-02
Files Summary

Total 19 files 4,172,075,878 4,172,075,878

Files Summary
File type # Files Total Size
raw 6 3,726,902,806 3,726,902,806
searchResultFile 13 445,173,072 445,173,072
File
file 목록
File Name Size Type Published Download mzMl QC file
evidence.txt 25,924,808 25,924,808 txt 2025-12-02
matchedFeatures.txt 0 0 txt 2025-12-02
modificationSpecificPeptides.txt 4,500,324 4,500,324 txt 2025-12-02
Mouse_heart_IMAC_DDA_1.raw 609,811,892 609,811,892 raw 2025-12-02
Mouse_heart_IMAC_DDA_2.raw 642,838,710 642,838,710 raw 2025-12-02
Mouse_heart_IMAC_DDA_3.raw 660,867,602 660,867,602 raw 2025-12-02
Mouse_heart_TiO2_DDA_1.raw 613,446,828 613,446,828 raw 2025-12-02
Mouse_heart_TiO2_DDA_2.raw 590,565,756 590,565,756 raw 2025-12-02
Mouse_heart_TiO2_DDA_3.raw 609,372,018 609,372,018 raw 2025-12-02
ms3Scans.txt 0 0 txt 2025-12-02
msms.txt 290,310,388 290,310,388 txt 2025-12-02
msmsScans.txt 107,099,366 107,099,366 txt 2025-12-02
mzRange.txt 2,951,764 2,951,764 txt 2025-12-02
Oxidation (M)Sites.txt 1,610,783 1,610,783 txt 2025-12-02
parameters.txt 4,337 4,337 txt 2025-12-02
peptides.txt 4,586,213 4,586,213 txt 2025-12-02
Phospho (STY)Sites.txt 5,857,241 5,857,241 txt 2025-12-02
proteinGroups.txt 2,324,739 2,324,739 txt 2025-12-02
summary.txt 3,109 3,109 txt 2025-12-02