단백체 데이터
KoBMAP_Mouse Heart Proteomics workflow comparison_Global_DIA
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AccessionKPX10000258
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Submission date2025-12-03
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Metadata export
Project Detail
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BioProject
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KAP241926 | ||||
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ProjectTitle
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Mouse heart proteomics workflow comparison for KoBMAP project | ||||
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Description
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To establish optimal analytical conditions for high-quality data generation, mouse heart tissue was used to evaluate different combinations of sample preparation and acquisition strategies. For global proteomics, In-solution, FASP, and S-trap digestion methods were assessed in combination with both DDA and DIA acquisition modes. For phosphoproteomics, TiO₂ and IMAC enrichment methods were evaluated together with DDA and DIA acquisition strategies. | ||||
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Keywords
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Submitter
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Kim Min-Sik , DGIST | ||||
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Publication
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Dataset Detail
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Dataset Title
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KoBMAP_Mouse Heart Proteomics workflow comparison_Global_DIA | ||||||||
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Submission Type
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Complete | ||||||||
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Species
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Mus musculus (Mouse) | ||||||||
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Sample type
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Disease
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Fractionation
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Digestion
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Quantification
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Modifications
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Acetyl (Protein N-term), Carbamidomethyl (C)
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Modifications
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Bottom-up proteomics
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MS instrument
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Thermo Scientific Orbitrap Exploris 480
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Sample processing protocol
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Data analysis protocol
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Supplementary information
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Announce Date
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2025-12-02 |
Files Summary
Total 22 files 10,781,924,280 10,781,924,280
| File type | # Files | Total Size |
|---|---|---|
| raw | 9 | 9,880,678,530 9,880,678,530 |
| searchResultFile | 13 | 901,245,750 901,245,750 |
| File Name | Size | Type | Published | Download | mzMl QC file | |
|---|---|---|---|---|---|---|
| Mouse_heart_FASP_DIA_1.raw | 906,520,478 906,520,478 | raw | 2025-12-02 | |||
| Mouse_heart_FASP_DIA_2.raw | 1,127,687,965 1,127,687,965 | raw | 2025-12-02 | |||
| Mouse_heart_FASP_DIA_3.raw | 985,966,635 985,966,635 | raw | 2025-12-02 | |||
| Mouse_heart_Insol_DIA_1.raw | 1,006,049,166 1,006,049,166 | raw | 2025-12-02 | |||
| Mouse_heart_Insol_DIA_2.raw | 1,011,653,605 1,011,653,605 | raw | 2025-12-02 | |||
| Mouse_heart_Insol_DIA_3.raw | 994,588,559 994,588,559 | raw | 2025-12-02 | |||
| Mouse_heart_Strap_DIA_1.raw | 1,275,895,034 1,275,895,034 | raw | 2025-12-02 | |||
| Mouse_heart_Strap_DIA_2.raw | 1,252,639,028 1,252,639,028 | raw | 2025-12-02 | |||
| Mouse_heart_Strap_DIA_3.raw | 1,319,678,060 1,319,678,060 | raw | 2025-12-02 | |||
| report-first-pass.gg_matrix.tsv | 525,913 525,913 | tsv | 2025-12-02 | |||
| report-first-pass.pg_matrix.tsv | 947,578 947,578 | tsv | 2025-12-02 | |||
| report-first-pass.pr_matrix.tsv | 15,736,005 15,736,005 | tsv | 2025-12-02 | |||
| report-first-pass.stats.tsv | 2,095 2,095 | tsv | 2025-12-02 | |||
| report-first-pass.tsv | 414,135,832 414,135,832 | tsv | 2025-12-02 | |||
| report-first-pass.unique_genes_matrix.tsv | 516,712 516,712 | tsv | 2025-12-02 | |||
| report.gg_matrix.tsv | 552,142 552,142 | tsv | 2025-12-02 | |||
| report.log.txt | 22,009 22,009 | txt | 2025-12-02 | |||
| report.pg_matrix.tsv | 973,926 973,926 | tsv | 2025-12-02 | |||
| report.pr_matrix.tsv | 16,340,946 16,340,946 | tsv | 2025-12-02 | |||
| report.stats.tsv | 2,114 2,114 | tsv | 2025-12-02 | |||
| report.tsv | 450,947,790 450,947,790 | tsv | 2025-12-02 | |||
| report.unique_genes_matrix.tsv | 542,688 542,688 | tsv | 2025-12-02 |