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Acetyl (Protein N-term), Carbamidomethyl (C), Oxidation (M)

Bottom-up proteomics

Thermo Scientific Orbitrap Exploris 240

After cell pellets were carefully washed in PBS buffer, cell pellets were lysed by 5% SDS. And performed by sonication at a setting of 20% energy and on/off cycle 3sec/3sec each for a total of 60 seconds. The lysis sample was heating 95°C for 10min and centrifuged at 12,000 g for 10 min. The supernatant was transferred to a new tube. Protein concentration was then measured using the BCA protein assay (BCA Protein Assay Kit, Pierce).

maxquat v2.4.0.0 - Mus Musculus, carbamidomethyl(C), FAIMS-MS2-reporter ion, use default values ​​for the rest. database - uniprot Mus Musculus, ficed modification - carbamidomethyl(C), acetyl(Nterm), variable modification - oxidation(M), type - FAIMS-MS2-reporter ion and use default values ​​for the rest

{"taxonomicType": "Model organism or animal", "sampleName": "NCD1 1;NCD1 2;NCD1 3;HFD1 1;HFD1 2;HFD1 3;NCD3 1;NCD3 2;NCD3 3;HFD3 1;HFD3 2;HFD3 3;NCD7 1;NCD7 2;NCD7 3;HFD7 1;HFD7 2;HFD7 3", "age": "12", "altitude": "", "biomaterialProvider": "N/A", "birthDate": "", "birthLocation": "", "breed": "C57BL/6", "breedingHistory": "", "breedingMethod": "", "broadScaleEnvironmentalContext": "", "cellLine": "", "cellSubtype": "", "cellType": "", "collectedBy": "", "collectionDate": "", "cultivar": "N/A", "cultureCollection": "", "deathDate": "", "depth": "", "description": "", "developmentStage": "13weeks", "disease": "", "diseaseStage": "", "ecotype": "N/A", "ethnicity": "", "genotype": "", "geographicLocation": "", "growthProtocol": "", "healthState": "", "heightOrLength": "", "host": "", "hostAge": "", "hostDescription": "", "hostDisease": "", "hostDiseaseOutcome": "", "hostDiseaseStage": "", "hostHealthState": "", "hostSex": "", "hostSubjectID": "", "hostTissueSampled": "", "identifiedBy": "", "isolate": "NCD-fed for 1day;NCD-fed for 1day;NCD-fed for 1day;HFD-fed for 1day;HFD-fed for 1day;HFD-fed for 1day;NCD-fed for 3day;NCD-fed for 3day;NCD-fed for 3day;HFD-fed for 3day;HFD-fed for 3day;HFD-fed for 3day;NCD-fed for 7day;NCD-fed for 7day;NCD-fed for 7day;HFD-fed for 7day;HFD-fed for 7day;HFD-fed for 7day", "isolationSource": "", "karyotype": "", "labHost": "", "latitudeAndLongitude": "", "matingType": "", "NCBITaxonomyID": "10090", "organism": "Mus musculus", "passageHistory": "", "pathotype": "", "phenotype": "", "population": "", "race": "", "referenceForBiomaterial": "", "relationshipToOxygen": "", "sampleCollectionDeviceOrMethod": "", "sampleMaterialProcessing": "", "sampleSize": "", "sampleType": "", "serotype": "", "serovar": "", "sex": "1", "sourceMaterialIdentifiers": "", "specimenVoucher": "N/A", "storageConditions": "", "strain": "", "studBookNumber": "", "subgroup": "", "subtype": "", "temperature": "", "tissue": "Brown adipose tissue", "treatment": "", "otherCharacteristics": "N/A"}