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Lipid extractions were performed by slightly modifying the Matyash method. 100% ice-cold methanol (300 µL) containing 0.1% butylated hydroxytoluene and H2O(90 uL) was added in the plasma(10 uL). After adding 1 mL of methyl-tert-butyl ether with 0.1% butylated hydroxytoluene, the samples were shaken at 20 to 23°C for 1 h. In total, 250 µL of water was added and vortexed for 10 min. Thereafter, phase separation was performed via centrifugation at 14,000g for 15 min at 4°C. For targeted lipidomics analysis, the upper (220 µL) and lower (110 µL) phases were pooled and dried using N2 purge. , Lipid extractions were performed by slightly modifying the Matyash method. 75% ice-cold methanol (400 µL) containing 0.1% butylated hydroxytoluene was added in the cell(10^6). After homogenizing the cells were removed using stainless steel beads and TissueLyser (QIAGEN, Germany’s Helden). After adding 1 mL of methyl-tert-butyl ether with 0.1% butylated hydroxytoluene, the samples were shaken at 20 to 23°C for 1 h. In total, 250 µL of water was added and vortexed for 10 min. Thereafter, phase separation was performed via centrifugation at 14,000g for 15 min at 4°C. For targeted lipidomics analysis, the upper (220 µL) and lower (110 µL) phases were pooled and dried using N2 purge. , Lipid extractions were performed by slightly modifying the Matyash method. 75% ice-cold methanol (400 µL) containing 0.1% butylated hydroxytoluene was added in the Liver tissue(5 mg). After adding 1 mL of methyl-tert-butyl ether with 0.1% butylated hydroxytoluene, the samples were shaken at 20 to 23°C for 1 h. In total, 250 µL of water was added and vortexed for 10 min. Thereafter, phase separation was performed via centrifugation at 14,000g for 15 min at 4°C. For targeted lipidomics analysis, the upper (220 µL) and lower (110 µL) phases were pooled and dried using N2 purge.

Samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS, LC-MS 8060, Shimadzu, Kyoto, Japan) with a Kinetex C18 column (100 x 2.1mm i.d., 2.6 μm particle size; Phenomenex, Torrance, CA, USA). Mobile phase A and B consisted of 10 mM ammonium acetate in water/methanol (1/9, v/v) and 10 mM ammonium acetate in methanol/isopropanol (1/1, v/v), respectively. The gradient elution condition was as follows: 30% B (0 min), 95% B (5 min), 95% B (15 min), 30% B (15.1 min) and 30% B (20 min). The sample injection volume was 5 μL and the flow rate was maintained at 0.2 mL/min. , Samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS, LC-MS 8060, Shimadzu, Kyoto, Japan) with a Kinetex C18 column (100 x 2.1mm i.d., 2.6 μm particle size; Phenomenex, Torrance, CA, USA). Mobile phase A and B consisted of 10 mM ammonium acetate in water/methanol (1/9, v/v) and 10 mM ammonium acetate in methanol/isopropanol (1/1, v/v), respectively. The gradient elution condition was as follows: 30% B (0 min), 95% B (5 min), 95% B (15 min), 30% B (15.1 min) and 30% B (20 min). The sample injection volume was 1 μL and the flow rate was maintained at 0.2 mL/min.

The mass operating conditions were as follows: desolvation temperature, 250 ℃; heat block temperature, 400 ℃; spray voltage 4 kV; drying gas (N2) flow rate, 10 L/min; collision gas, argon; nebulizing gas (N2) flow rate, 3 L/min; collision gas pressure, 230 kPa; and detector voltage, 1.66 kV.