Experiment
KAE21051310
Experiment Title
Illumina NovaSeq 6000 paired-end Sequencing of KAS20000430
(DRX649299)
Common Fields
|
Organism
|
Homo sapiens | 9606 |
|---|---|
|
Submission date
|
2021-03-22 |
Accession
|
Runs
|
All runs [96] |
|---|---|
|
Experiments
|
All experiments [96] |
|
BioSamples
|
All samples [60] |
|
BioProject
|
KAP200004 |
Library
|
Library name
|
SNU449_ATAC_rep2_ATAC-Seq
|
|---|---|
|
Instrument model
|
Illumina NovaSeq 6000
|
|
Strategy
|
ChIA-PET
|
|
Source
|
GENOMIC
|
|
Selection
|
other
|
|
Layout
|
paired
|
|
Construction protocol
|
Total 50,000 SNU449 cells were washed two times with 50ul cold PBS, and resuspended with 50ul cold lysis buffer10 mM Tris-Cl, pH7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40. After centrifugation at 2,000 rpm for 10 min at 4?? nuclei were incubated in fresh 25ul TD Buffer 10mM Tris-HCl pH8.0, 5mM MgCl2, 10% dimethylformamide with 2ul Tn5 Transposase and 23ul DW at 37degree Celsius for 30min. QIAquick PCR purification kitQiagen; cat. no. 28106 was used to purify DNA fragments. We used HiFi HotStart ReadyMix KAPA; KK2601 for library amplification following provided manual except number of PCR cycle: we performed 15 cycle for PCR amplification. The amplified library was purified with a QIAquick PCR purification kit Qiagen; cat. no. 28106 and sequenced using a NovaSeq2500 system150bp with the paired method.
|
| Run | File Name | Size | Type | Published | Download | |
|---|---|---|---|---|---|---|
| KRA: KAR21051310 , DRA: DRR669197 | READ_1.fq.gz | 3,995,098,857 3,995,098,857 | fastq | 2021-12-03 | ||
| KRA: KAR21051310 , DRA: DRR669197 | READ_2.fq.gz | 3,606,995,207 3,606,995,207 | fastq | 2021-12-03 |