Experiment
KAE21040540
Experiment Title
Illumina HiSeq 4000 paired-end Sequencing of KAS20000264
(DRX649315)
Common Fields
|
Organism
|
Homo sapiens | 9606 |
|---|---|
|
Submission date
|
2021-03-22 |
Accession
|
Runs
|
All runs [96] |
|---|---|
|
Experiments
|
All experiments [96] |
|
BioSamples
|
All samples [60] |
|
BioProject
|
KAP200004 |
Library
|
Library name
|
T47D_TCPS_Deep_rep2_GENOMIC
|
|---|---|
|
Instrument model
|
Illumina HiSeq 4000
|
|
Strategy
|
RAD-Seq
|
|
Source
|
GENOMIC
|
|
Selection
|
other
|
|
Layout
|
paired
|
|
Construction protocol
|
In-situ Hi-C was performed as previously described Rao et al., 2014. Briefly: T47D cells were crosslinked with 1% formaldehyde; nuclei were isolated; chromatin was digested with MboI R0147, NEB; 5??ends were filled by incorporation of biotinylated-dCTP 19524-016, Life Technologies; proximity ligation, reverse-crosslinking, and DNA shearing were performed; biotinylated junctions were isolated with streptavidin beads 65601, Life Technologies; and DNA libraries were prepared. Each Hi-C library was amplified for six cycles and then subjected to deep sequencing on a Hiseq4000 Illumina platform. The libraries were generated from two sets of biological replicates.
|
| Run | File Name | Size | Type | Published | Download | |
|---|---|---|---|---|---|---|
| KRA: KAR21040540 , DRA: DRR669213 | READ_1.fq.gz | 34,249,182,728 34,249,182,728 | fastq | 2021-12-03 | ||
| KRA: KAR21040540 , DRA: DRR669213 | READ_2.fq.gz | 39,016,895,640 39,016,895,640 | fastq | 2021-12-03 |