Experiment
KAE21040050
Experiment Title
Illumina HiSeq 2500 paired-end Sequencing of KAS20000285
(DRX649272)
Common Fields
|
Organism
|
Homo sapiens | 9606 |
|---|---|
|
Submission date
|
2021-03-22 |
Accession
|
Runs
|
All runs [96] |
|---|---|
|
Experiments
|
All experiments [96] |
|
BioSamples
|
All samples [60] |
|
BioProject
|
KAP200004 |
Library
|
Library name
|
T47D_rep1_GENOMIC
|
|---|---|
|
Instrument model
|
Illumina HiSeq 2500
|
|
Strategy
|
ChIA-PET
|
|
Source
|
GENOMIC
|
|
Selection
|
other
|
|
Layout
|
paired
|
|
Construction protocol
|
Total 50,000 BT549 cells were washed two times with 50ul cold PBS, and resuspended with 50ul cold lysis buffer10 mM Tris-Cl, pH7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40. After centrifugation at 2,000 rpm for 10 min at 4?? nuclei were incubated in fresh 25ul TD Buffer 10mM Tris-HCl pH8.0, 5mM MgCl2, 10% dimethylformamide with 2.5ul Tn5 Transposase and 23ul DW at 37degree Celsius for 30min. QIAquick PCR purification kitQiagen; cat. no. 28106 was used to purify DNA fragments. We used HiFi HotStart ReadyMix KAPA; KK2601 for library amplification following provided manual except number of PCR cycle: we performed 15 cycle for PCR amplification. The amplified library was purified with a QIAquick PCR purification kit Qiagen; cat. no. 28106 and sequenced using a Hiseq2500 system50bp with the paired method.
|
| Run | File Name | Size | Type | Published | Download | |
|---|---|---|---|---|---|---|
| KRA: KAR21040050 , DRA: DRR669170 | READ_1.fq.gz | 1,208,285,141 1,208,285,141 | fastq | 2021-12-03 | ||
| KRA: KAR21040050 , DRA: DRR669170 | READ_2.fq.gz | 1,249,669,484 1,249,669,484 | fastq | 2021-12-03 |