염기서열
Miscanthus sacchariflorus BD002137 plastid:chloroplast , genome
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AccessionSN01000032
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Submission date2025-09-12
Project Detail
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BioProject
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KAP241606 |
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프로젝트의 영문 제목
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Complete chloroplast genome of Crop Wild Relatives(CWR)
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프로젝트의 영문 설명
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Crop Wild Relatives (CWR) are important genetic resources that provide valuable traits for crop improvement and cultivar development. This study aims to establish a fundamental dataset for cataloging major CWR distributed in Korea and analyzing their phylogenetic relationships. The chloroplast is a key organelle in plant cells that plays an essential role in photosynthesis and various metabolic pathways. In particular, the chloroplast genome evolves more slowly than the nuclear genome and is maternally inherited, making it a reliable marker for species classification and phylogenetic analysis. In this study, we aim to sequence and analyze the complete chloroplast genomes of major crop wild relatives to investigate their phylogenetic relationships and taxonomic characteristics. The resulting data will serve as a valuable resource for future research on the conservation of genetic diversity, cultivar development, and the discovery of useful genes in crop wild relatives.
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등록자의 영문 이름
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Chae Sun Na
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BioSample
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BioSample Accession ID
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KAS24121418 |
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BioSample name
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BD002137
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Organism
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Miscanthus sacchariflorus
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NCBItaxonomyID
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183675
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Strain
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Breed
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Cultivar
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not applicable
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Isolate
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not applicable
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Ecotype
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missing:not collected
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Host
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Lab Host
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Isolation Source
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Sample Division
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Plants and Fungi
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DataSet
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Name of submitter's organization
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Baekdudaegan National Arboretum
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Department of submitter
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Wild Plant Seed Division
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Country of submitter
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대한민국
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Street address
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1501 Chunyang-ro, Chunyang-myeon
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City
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Bonghwa-gun
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State / Province / Region
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Gyeongsangbuk-do
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Zip Code (Postal Code)
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36209
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Contact person information
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Publication status
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Unpublished
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Publication
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Reference title
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Confirm update of existing submission
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No
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Existing KNA Accession ID
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Sequence Authors
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Sample processing protocol
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NucleoSpin Plant II Kit (Macherey-Nagel, Germany)
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Data processing protocol
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Paired-end sequencing libraries were prepared using the TruSeq Nano DNA Kit (Illumina, San Diego, CA, USA), and sequencing was performed on the Illumina NovaSeq 6000 platform. Adapter removal and quality filtering (Phred score <20) were conducted with Trimmomatic. De novo assembly was performed with GetOrganelle and CLC Assembly Cell v4.2.1, followed by reference-guided assembly. Genome annotation was carried out using GeSeq and manually curated with Geneious Prime v2025.1.2.
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Supplementary information
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The complete chloroplast genome sequences have been deposited in NCBI GenBank (Accession No. PX334449)
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Release Protocol?
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No
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Did you submit KRA data for this Nucleotide submission?
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KRA Accession ID
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Assembly date
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Sequencing platform - KRA Run
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Sequencing platform
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Sequencing platform
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Assembly method
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Haplotype phasing method
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Assembly name
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Molecule Type
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genomic DNA
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Topology
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Circular
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Organelle/Location information
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plastid:chloroplast
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Status
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Feature Information
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Genome Accession
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Annotation file format
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Genbank(.gb)
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Total length
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32702
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Total contigs
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1
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Scaffolds or chromosomes Total length
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Scaffolds or chromosomes Total contigs
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| File Name | Size | Type | Published | Download | |
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| Miscanthus sacchariflorus BD002137 plastid:chloroplast , genome.fasta | 32,768 32,768 | fasta | 2025-11-04 |