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KoBMAP_Mouse Heart Proteomics workflow comparison_Global_DDA
  • Accession
    KPX10000257
  • Submission date
    2025-12-02
  • Metadata export
    XML Download
Project Detail
Dataset detail - Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol
BioProject
 KAP241926
ProjectTitle
 Mouse heart proteomics workflow comparison for KoBMAP project
Description
 To establish optimal analytical conditions for high-quality data generation, mouse heart tissue was used to evaluate different combinations of sample preparation and acquisition strategies. For global proteomics, In-solution, FASP, and S-trap digestion methods were assessed in combination with both DDA and DIA acquisition modes. For phosphoproteomics, TiO₂ and IMAC enrichment methods were evaluated together with DDA and DIA acquisition strategies.
Keywords
  
Submitter
 Kim Min-Sik , DGIST
Publication
Publication
PubMed ID DOI
   
Dataset Detail
Dataset detail - Description, Keywords, Principal investigato, Pubmed ID, Doi, Dataset Tilte, Species, Sample type, Disease, Offline, SCX, 16 fractions, Trypsin, Quantification, Modifications, Experiment type, MS instrument, Experimental protocol, Data analysis protocol
Dataset Title
 KoBMAP_Mouse Heart Proteomics workflow comparison_Global_DDA
Submission Type
 Complete
Species
 Mus musculus (Mouse)
Sample type
Sample type
Body fluid Tissue Cell Others
heart
Disease
Disease
Fractionation
Fractionation
Method Separation mode Number of fractions
Digestion
 Trypsin
Quantification
Quantification
Labeling Labeling Child Plex
()
Modifications
Acetyl (Protein N-term), Carbamidomethyl (C), Oxidation (M)
Modifications
Bottom-up proteomics
MS instrument
Thermo Scientific Orbitrap Exploris 480
Sample processing protocol
Data analysis protocol
Search parameters
Supplementary information
 
Announce Date
 2025-12-01
Files Summary

Total 21 files 16,063,486,954 16,063,486,954

Files Summary
File type # Files Total Size
raw 9 13,252,812,334 13,252,812,334
searchResultFile 12 2,810,674,620 2,810,674,620
File
file 목록
File Name Size Type Published Download mzMl QC file
evidence.txt 122,517,742 122,517,742 txt 2025-12-01
matchedFeatures.txt 0 0 txt 2025-12-01
modificationSpecificPeptides.txt 16,312,736 16,312,736 txt 2025-12-01
Mouse_heart_FASP_DDA_1.raw 1,468,714,992 1,468,714,992 raw 2025-12-01
Mouse_heart_FASP_DDA_2.raw 1,297,084,514 1,297,084,514 raw 2025-12-01
Mouse_heart_FASP_DDA_3.raw 1,445,193,113 1,445,193,113 raw 2025-12-01
Mouse_heart_Insol_DDA_1.raw 1,380,325,874 1,380,325,874 raw 2025-12-01
Mouse_heart_Insol_DDA_2.raw 1,395,823,969 1,395,823,969 raw 2025-12-01
Mouse_heart_Insol_DDA_3.raw 1,405,206,615 1,405,206,615 raw 2025-12-01
Mouse_heart_Strap_DDA_1.raw 1,650,684,121 1,650,684,121 raw 2025-12-01
Mouse_heart_Strap_DDA_2.raw 1,579,421,871 1,579,421,871 raw 2025-12-01
Mouse_heart_Strap_DDA_3.raw 1,630,357,265 1,630,357,265 raw 2025-12-01
ms3Scans.txt 0 0 txt 2025-12-01
msms.txt 2,217,765,950 2,217,765,950 txt 2025-12-01
msmsScans.txt 422,178,540 422,178,540 txt 2025-12-01
mzRange.txt 4,561,161 4,561,161 txt 2025-12-01
Oxidation (M)Sites.txt 1,639,120 1,639,120 txt 2025-12-01
parameters.txt 4,295 4,295 txt 2025-12-01
peptides.txt 18,252,588 18,252,588 txt 2025-12-01
proteinGroups.txt 7,438,537 7,438,537 txt 2025-12-01
summary.txt 3,951 3,951 txt 2025-12-01