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The culture broth was extracted twice with ethyl acetate (EtOAc) in a 1:1 ratio.

LC separations were carried out on an Acquity UPLC BEH C18 column (2.1 x 100 mm, 1.7 µm). Column temperature and flow rate were set to 40 °C and 0.3 ml/min, respectively. The mobile phases used were 0.1 % formic acid in water (A) and acetonitrile (B). The linear gradients were as follows: 10–100% B for 12 min followed by a 3 min washout phase at 100 % B and a 3 min re-equilibration phase at 10 % B, successively. The injection volume of the sample was set to 2.0 μl.

LC-MS/MS data used in this study were acquired using a Waters Acquity UPLC system (Waters Co., Milford, MA, USA) coupled to a Waters VION IMS Q/TOF mass spectrometer (Waters MS Technologies, Manchester, UK) equipped with an electrospray ionization (ESI) interface. MS/MS analyses were performed in MSE data-independent acquisition mode for negative ions. , LC-MS/MS data used in this study were acquired using a Waters Acquity UPLC system (Waters Co., Milford, MA, USA) coupled to a Waters VION IMS Q/TOF mass spectrometer (Waters MS Technologies, Manchester, UK) equipped with an electrospray ionization (ESI) interface. MS/MS analyses were performed in MSE data-independent acquisition mode for positive ions.