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BioProject
할로젠 첨가 배양과 대사체학을 활용한 진균 유래 천연물 발굴
  • Accession
    KAP241860
  • Submission date
    2025-11-17
Technique type
Mass spectrometry (MS)
Assay Type
Chromatography (MS-based)
Species
Aspergillus brunneoviolaceus , Aspergillus calidoustus , Aspergillus chevalieri , Aspergillus clavatus , Aspergillus fumigatus , Aspergillus gracilis , Aspergillus iizukae , Aspergillus lentulus , Aspergillus montevidensis , Aspergillus nidulans , Aspergillus niger , Aspergillus niveus , Aspergillus ruber , Aspergillus sydowii , Aspergillus terreus , Aspergillus tubingensis , Aspergillus unguis , Aspergillus westerdijkiae , Penicillium allii , Penicillium aurantioviolaceum , Penicillium bilaiae , Penicillium chrysogenum , Penicillium coprophilum , Penicillium daejeonium , Penicillium fellutanum , Penicillium janthinellum , Penicillium jejuense , Penicillium limosum , Penicillium mallochii , Penicillium nalgiovense , Penicillium polonicum , Penicillium radicicola , Penicillium roseomaculatum , Penicillium sp.17 , Penicillium sp.97 , Penicillium spinulosum , Penicillium steckii , Penicillium trzebinskii , Penicillium velutinum , Penicillium yarmokense
샘플 유형
Microbe
추출 프로토콜
Aspergillus strains were cultured in defined media with 100 uM NaBr for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Aspergillus strains were cultured in defined media with 100 uM NaCl for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Aspergillus strains were cultured in defined media for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Aspergillus strains were cultured in defined media with 100 uM NaI for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Penicillium strains were cultured in defined media with 100 uM NaBr for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Penicillium strains were cultured in defined media with 100 uM NaCl for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Penicillium strains were cultured in defined media for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Penicillium strains were cultured in defined media with 100 uM NaI for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate. , Penicllium strains were cultured in defined media with 100 uM NaBr for 15 days then each of them was added EtOAc, sonicate for 2 hrs and evaporate.
크로마토그래피 프로토콜
LC separations were carried out on an Acquity UPLC BEH C18 column (2.1 x 100 mm, 1.7 µm). Column temperature and flow rate were set to 40 °C and 0.3 ml/min, respectively. The mobile phases used were 0.1 % formic acid in water (A) and acetonitrile (B). The linear gradients were as follows: 10–100% B for 12 min followed by a 3 min washout phase at 100 % B and a 3 min re-equilibration phase at 10 % B, successively. The injection volume of the sample was set to 2.0 μl.
질량분석 프로토콜
LC-MS/MS data used in this study were acquired using a Waters Acquity UPLC system (Waters Co., Milford, MA, USA) coupled to a Waters VION IMS Q/TOF mass spectrometer (Waters MS Technologies, Manchester, UK) equipped with an electrospray ionization (ESI) interface. MS/MS analyses were performed in MSE data-independent acquisition mode for positive ions.