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50 µL aliquot of the sera were transferred to a 1.5 mL microcentrifuge tubes. Serum samples were stored at −80 °C freezer prior to untargeted metabolomic analysis. On the day of analysis, samples were slowly thawed at 4 °C in a refrigerator. Then, 150 µL of cold ACN and 50 µL of cold methanol were added to each sample tube. After vortexing for 1 min, samples were stored in a refrigerator for 45 min to induce protein precipitation. Subsequently, the samples were vortexed for 1 min. Centrifugation was performed at 16,022 x g for 10 min at 4 °C. The supernatant was filtered through a 0.22 µm PTFE filter and transferred into LC sample vial. , 50 µL aliquot of the sera were transferred to a 1.5 mL microcentrifuge tube. Serum samples were stored at −80 °C freezer prior to untargeted lipidomic analysis. On the day of analysis, samples were slowly thawed at 4 °C in a refrigerator. Then, 200 µL of cold isopropyl alcohol was added to each sample tube. After vortexing for 1 min, samples were stored in a refrigerator for 10 min to induce protein precipitation. Subsequently, the samples were vortexed for 1 hour. Centrifugation was performed at 16,022 x g for 10 minutes at 4 °C. The supernatant was filtered through a 0.22 µm PTFE filter and dried under a stream of nitrogen gas for subsequent reconstitution of lipid samples. The reconstitution solvent was prepared using the same ratio as the initial LC mobile phase.

We used Acquity UPLC BEH HILIC (2.1 mm × 100 mm, Waters, Milford, MA, USA) column. An oven temperature, flow rate, and injection volume were 30 °C, 0.3 mL/min, and 1 μl, respectively. Mobile phase A (100% dH2O with 5 mM ammonium formate and 0.2% formic acid) and mobile phase B (100% ACN with 0.2% formic acid) were used for gradient elution solvents. The gradient started at 95% B and was held for 2 min in the positive ion mode. Then, B was linearly decreased to 80% at 11 min and held until 12 min. , We used Acquity UPLC BEH HILIC (2.1 mm × 100 mm, Waters, Milford, MA, USA) column. An oven temperature, flow rate, and injection volume were 30 °C, 0.3 mL/min, and 1 μL, respectively. Mobile phase A (100% dH2O with 5 mM ammonium acetate and 0.05% ammonium hydroxide solution) and mobile phase B (100% ACN with 0.05% ammonium hydroxide solution) were used for gradient elution. The gradient started at 95% B and was held for 2 min in the negative ion mode. Then, B was linearly decreased to 90% at 7 min, 85% at 7.1 min, 80% at 11 min and held until 12 min. , We used Acquity UPLC HSS T3 (2.1 mm × 150 mm, Waters, Milford, MA, USA) column. An oven temperature, flow rate, and injection volume were 40 °C, 0.3 mL/min, and 5 μL, respectively. Mobile phase A (100% dH2O with 1 mM ammonium formate and 0.1% formic acid) and mobile phase B (3:1:1 ratio of isopropyl alcohol, methanol, and ACN with 1 mM ammonium formate and 0.1% formic acid) were used for gradient elution solvents. The gradient started at 25% B and was held for 1 min both positive and negative ion modes. Then, B was linearly increased to 35% at 9 min, 70% at 10 min, 99% at 35 min and held until 40 min.

We use X500R QTOF (AB SCIEX, Marlborough, MA, USA) mass spectrometry. In the positive ion mode, ion spray voltage, curtain gas, ion source gas 1 and 2, ion source temperature, CAD gas, and declustering potential (DP) were set to 4,500 V, 35 psi, 50 psi, 500 °C, 7 psi, and 60 V, respectively. The m/z scan range was set to 50 ~ 900 Da. Sequential windowed acquisition of all theoretical fragment ions (SWATH) was used to acquire MS/MS data. Dynamic CE parameters were 8 ~ 50 V, and CE spread was 15. , We use X500R QTOF (AB SCIEX, Marlborough, MA, USA) mass spectrometry. In the negative ion mode, ion spray voltage, curtain gas, ion source gas 1 and 2, ion source temperature, CAD gas, and declustering potential (DP) were also set to −4,500 V, 35 psi, 50 psi, 500 °C, 7 psi, and −60 V, respectively. The m/z scan range was set to 50 ~ 900 Da. Sequential windowed acquisition of all theoretical fragment ions (SWATH) was used to acquire MS/MS data. Dynamic CE parameters were -8 ~ -50 V, and CE spread was 15. , We use X500R QTOF (AB SCIEX, Marlborough, MA, USA) mass spectrometry. In the positive ion mode, ion spray voltage, curtain gas, ion source gas 1 and 2, ion source temperature, CAD gas, and declustering potential (DP) were set to 4,500 V, 35 psi, 45 psi, 450 °C, 7 psi, and 60 V, respectively. The m/z scan range was set to 50 ~ 1100 Da. Sequential windowed acquisition of all theoretical fragment ions (SWATH) was used to acquire MS/MS data. Dynamic CE parameters were 20 ~ 50 V, and CE spread was 5. , We use X500R QTOF (AB SCIEX, Marlborough, MA, USA) mass spectrometry. In the negative ion mode, ion spray voltage, curtain gas, ion source gas 1 and 2, ion source temperature, CAD gas, and declustering potential (DP) were also set to −3,500 V, 35 psi, 45 psi, 450 °C, 7 psi, and −60 V, respectively. The m/z scan range was set to 50 ~ 1100 Da. Sequential windowed acquisition of all theoretical fragment ions (SWATH) was used to acquire MS/MS data. Dynamic CE parameters were -20 ~ -50 V, and CE spread was 5.