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10-12mg of each sample was transferred into a disposable insert tube. The residual space in the tube was filled with deuterium oxide (99.9 atom % D). And then, the disposable insert tube was inserted into a zirconium HR-MAS rotor (4mm outer diameter).

10-12mg of each sample was transferred into a disposable insert tube. The residual space in the tube was filled with deuterium oxide (99.9 atom % D). And then, the disposable insert tube was inserted into a zirconium HR-MAS rotor (4mm outer diameter). The samples were maintained in a refrigerated tray at - 20˚C before the analysis.

All HR-MAS 1H NMR spectra of heart sample were acquired on a Bruker Avance III 700 MHz NMR spectrometer (Bruker BioSpin,Germany) with a Bruker 4 mm TXI HR-MAS probe with z-gradients at 278 K and a spinning rate of 6 kHz using CPMG spin-echo pulse sequence. For all spectra of each heart sample, 128 transients were acquired with total T2 filter time of 37.8 ms, 32 k data points, a spectral width of 14,097.744 Hz, and the relaxation delay of 4 s, and acquisition time of 1.162 s.