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BioProject
생리활성 모노테르펜 인돌 알칼로이드 발굴을 위한 협력적 식물대사체학 연구
  • Accession
    KAP240384
  • Submission date
    2023-02-28
Technique type
Mass spectrometry (MS)
Assay Type
Chromatography (MS-based)
Species
NA , Melodinus cochinchinensis , Urceola napeensis , Chonemorpha fragrans , Melodinus fusiformis , Adina pilulifera , Aganosma cymosa , Aidia canthioides , Aidia cochinchinensis , Aidia henryi , Aidia oxyodonta , Alibertia edulis , Alleizettella leucocarpa , Alstonia macrophylla , Alstonia mairei , Alyxia annamensis , Alyxia siamensis , Amaioua pedicellata , Amalocalyx microlobus , Amphidasya longicalycina , Amphineurion marginatum , Anodendron affine , Apocynum venetum , Apocynum venetum subsp. lancifolium , Arachnothryx buddleioides , Beaumontia grandiflora , Bertiera guianensis , Brachytome wallichii , Buddleja officinalis , Caelospermum truncatum , Campestigma purpurea , Canthiumera robusta , Carissa spinarum , Cascabela thevetia , Catharanthus roseus , Catunaregam tomentosa , Cephaelis lecomtei(unresolved) , Cephalanthus occidentalis , Cerbera odollam , Ceriscoides turgida , Chassalia curviflora , Chimarrhis parviflora , Chomelia spinosa , Chonemorpha verrucosa , Cinchona officinalis , Coffea arabica , Coffea liberica , Condaminea corymbosa , Coptosapelta flavescens , Cosmostigma cordatum , Coussarea curvigemmia , Cynanchum corymbosum , Cynanchum pulchellum , Cynanchum purpureum , Cyperus digitatus , Dioecrescis erythroclada , Diplospora dubia , Discospermum abnorme , Discospermum quocensis , Dyera costulata , Ehretia dicksonii , Elaeagia glossostipula , Exallage microcephala , Fagraea auriculata , Fagraea cf. , Fagraea crenulata , Fagraea fragrans , Fagraea racemosa , Forsteronia myriantha , Galium asperfolium , Galium trifloriforme , Galium maximowiczii  , Galium platygalium , Gardenia jasminoides , Gardenia philastrei , Gardenia sootepensis , Gardneria linifolia , Geophila repens , Gomphocarpus fruticosus , Greenea corymbosa , Guettarda combsii , Guettarda hirsuta , Gynochthodes umbellata , Haldina cordifolia , Hamelia macrantha , Hamelia patens , Hedyotis cf. , Hedyotis scandens , Hoffmannia nicotianifolia , Hoffmannia psychotriifolia , Holarrhena curtisii , Hoya obovata(unresolved) , Hunteria zeylanica , Hydnophytum formicarum , Hymenodictyon flaccidum , Hypobathrum racemosum , Isertia laevis , Ixora balansae , Ixora flavescens , Ixora henryi , Ixora nigricans , Ixora pierrei , Kailarsenia godefroyana , Kibatalia laurifolia , Kopsia singapurensis , Ladenbergia oblongifolia , Lasianthus japonicus , Lasianthus saprosmoides , Lasianthus verticillatus , Lasianthus fordii , Lasianthus trichophlebus , Leptodermis kumaonensis , Leptodermis ovata , Leuconotis eugeniifolia , Luculia gratissima , Macrocnemum roseum , Marsdenia hirta , Melodinus honbaensis , Melodinus orientalis , Melodinus cochinchinensis  , Mesechites acuminatus , Metadina trichotoma , Mitracarpus hirtus , Mitragyna hirsuta , Mitragyna rotundifolia , Mitragyna hirsuta , Morinda angustifolia , Morinda panamensis , Morindopsis capillaris , Mortoniella pittieri , Mussaenda cambodiana , Mussaenda glabra , Mussaenda squiresii , Mycetia effusa , Mycetia faberi , Myriopteron extensum , Myrmecodia pendens , Nauclea orientalis , Neohymenopogon parasiticus , Neolamarckia cadamba , Neonauclea sessilifolia , Neonauclea purpurea , Nertera granadensis , Nostolachma odorata , Notopleura polyphlebia , Ocotea veraguensis , Odontadenia puncticulosa , Oldenlandia auricularia , Oldenlandia corymbosa , Oldenlandia auricularia , Oldenlandia corymbosa , Oldenlandia diffusa , Ophiorrhiza alatiflora , Ophiorrhiza baviensis , Ophiorrhiza japonica , Oxyceros longiflorus , Oxyceros vidalii , Oxystelma esculentum , Paederia foetida , Paederia lanuginosa , Paederia foetida , Pagiantha curvisepala , Palicourea amethystina , Palicourea azurea , Palicourea galeottiana , Palicourea padifolia , Paralasianthus lowianus , Pavetta bauchei , Pavetta cambodiensis , Pavetta canescens , Pennisetum purpureum , Pentagonia gomez-lauritoi , Pentagonia hirsuta , Pentalinon luteum , Pentatropis pierrei(unresolved) , Plumeria obtusa var. sericifolia , Pogonopus exsertus , Porterandia laxiflora , Posoqueria latifolia , Potalia turbinata , Pottsia laxiflora , Prismatomeris tetrandra , Psychotria adenophylla , Psychotria balansae , Psychotria cephalophora , Psychotria convergens , Psychotria fleuryi , Psychotria poeppigiana , Psychotria psychotriifolia , Psychotria sarmentosa , Psychotria serpens , Psychotria sylvivaga , Psychotria tutcheri , Psychotria cuspidata , Psydrax pergracilis , Rauvolfia littoralis , Rauvolfia micrantha , Rauvolfia tetraphylla , Rauvolfia verticillata , Ridsdalea eucodon  , Rogiera nicaraguensis , Rubia chinensis , Rubia cordifolia , Rubia sylvatica , Sabicea villosa , Saprosma chevalieri , Saprosma verrucosum , Serissa japonica , Silvianthus bracteatus subsp. tonkinensis , Singaporandia macrophylla , Spermacoce alata , Spermacoce ovalifolia , Spermacoce articularis , Spigelia anthelmia , Spiradiclis caespitosa , Spirolobium cambodianum , Streptocaulon wallichii , Strophanthus caudatus , Strychnos angustiflora , Strychnos axillaris , Strychnos cathayensis , Strychnos chlorantha , Strychnos minor var. thorelli , Strychnos nitida , Strychnos ovata , Strychnos sonlaensis , Strychnos thorelii , Strychnos vanprukii , Tabernaemontana bovina , Tabernaemontana divaricata , Tabernaemontana glabra , Tabernaemontana granatum , Tabernaemontana pauciflora , Tabernaemontana bovina , Tamilnadia uliginosa , Tarenna annamensis , Tarenna hoaensis , Tarennoidea wallichii , Timonius arboreus , Timonius flavescens , Toxocarpus villosus , Toxocarpus wightianus , Trachelospermum asiaticum , Tylophora indica , Uncaria gambir , Uncaria lanosa var. glabrata , Uncaria scandens , Urceola laevigata , Urceola micrantha , Urceola micrantha , Urceola minutiflora , Urophyllum lecomtei , Urophyllum longifolium , Urophyllum streptopodium , Vallaris solanacea , Vidalasia tonkinensis , Vincetoxicum flexuosum , Vincetoxicum hainanense , Wendlandia puberula , Wendlandia thorelii , Willughbeia edulis , Willughbeia edulis , Wrightia arborea , Wrightia pubescens , Wrightia religiosa , Wrightia dubia , Zygostelma benthamii(unresolved)
샘플 유형
Plant or fungi
추출 프로토콜
acid-base extraction of alkaloids , NA
크로마토그래피 프로토콜
LC separations were carried out on an Acquity UPLC BEH C18 column (2.1 x 100 mm, 1.7 µm). Column temperature and flow rate were set to 40 °C and 0.3 ml/min, respectively. The mobile phases used were 0.1 % formic acid in water (A) and acetonitrile (B). The linear gradients were as follows: 10–100% B for 12 min followed by a 3 min washout phase at 100 % B and a 3 min re-equilibration phase at 10 % B, successively. The injection volume of the sample was set to 2.0 μl.
질량분석 프로토콜
LC-MS/MS data used in this study were acquired using a Waters Acquity UPLC system (Waters Co., Milford, MA, USA) coupled to a Waters VION IMS Q/TOF mass spectrometer (Waters MS Technologies, Manchester, UK) equipped with an electrospray ionization (ESI) interface. MS/MS analyses were performed in MSE data-independent acquisition mode for positive ions.