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전자현미경 데이터
TLR1-TLR2 heterodimer 의 CryoEM 구조
  • Accession
    KIX10061617
  • Submission date
    2026-09-14
Project Detail
Dataset detail - Accession, 프로젝트의 영문 제목, 프로젝트의 국문 제목, 프로젝트의 영문 설명, 프로젝트의 국문 설명
Accession
 KAP242546
프로젝트의 영문 제목
Cryo-EM Characterization of Ligand-Induced High-Order Toll-Like Receptor 2 (TLR2) Heteromer Clusters and Activation Mechanisms
프로젝트의 국문 제목
초저온 전자현미경을 이용한 리간드 유도 TLR2 이종다량체 고차 클러스터의 구조 분석 및 활성화 메커니즘 규명
프로젝트의 영문 설명
This project aims to elucidate the molecular architecture and immune signaling mechanisms of high-order clusters formed by Toll-like receptor 2 (TLR2) heteromers with coreceptors (TLR1 or TLR6) upon ligand induction. TLR2 recognizes diverse pathogen-derived lipopeptides, and the spatial clustering of receptor complexes induced by ligand binding is essential for recruiting downstream adaptor proteins (MyD88/MAL) and triggering the inflammatory signaling cascade. In this study, we determined the high-resolution supramolecular assembly of TLR2 heterodimers in complex with synthetic lipopeptide ligands using single-particle cryo-electron microscopy (Cryo-EM). Through this, we characterized the interfacial interactions between heteromeric complexes, ligand-induced conformational transitions, and the organizational principles of the supramolecular signaling platform. This work unveils the structural mechanism of TLR2-mediated innate immune activation, providing a precise molecular blueprint for the development of vaccine adjuvants and therapeutics for inflammatory disorders.
프로젝트의 국문 설명
본 프로젝트는 선천면역 수용체인 Toll-like receptor 2(TLR2)가 보조 수용체(TLR1 또는 TLR6)와 형성하는 이종다량체(heteromer)의 리간드 유도 고차 집적(clustering) 구조와 면역 신호전달 기전을 규명하기 위해 수행되었습니다. TLR2는 병원체 유래의 다양한 지질단백질(lipopeptide)을 인식하며, 리간드 결합 후 유도되는 수용체 복합체 간의 공간적 클러스터 형성은 하위 어댑터 단백질(MyD88/MAL) 동원과 염증 반응 캐스케이드 개시에 필수적입니다. 본 연구에서는 TLR2 이종이량체와 합성 지질펩타이드 리간드 복합체의 고차원 조립 상태를 초저온 전자현미경(Cryo-EM) 단입자 분석 기법을 통해 고해상도로 규명하였습니다. 이를 통해 이종 복합체 간 상호작용 계면, 리간드 결합에 따른 입체구조적 전이, 그리고 초분자 신호전달 플랫폼 형성 원리를 분석하였습니다. TLR2 매개 선천면역 활성화의 구조적 기전을 밝히고, 면역보조제 및 염증성 질환 치료제 개발을 위한 정밀한 분자 청사진을 제공합니다.
메타데이터 정보
메타데이터 입력
장비 유형
TEM
Name
김지원
Email address
mokona5@postech.ac.kr
Role assignment
Researcher
Role notation
Sample preparation, Raw data collection, Image analysis, Computational image processing
Contributor
Contributor name
이지오
Contributor email
Contributor role assignment
Principal investgator
Contributor role notation
typeOfInstrument
TEM
Representative image data file
Title - English
CryoEM structure of TLR1-TLR2 heterodimer
Title - Korean
TLR1-TLR2 heterodimer 의 CryoEM 구조
Experimental method
Single particle analysis
Raw Data
Data upload
Pixel size
Pixel size
Pixel units
Accelerating Voltage
Working distance
Emission current
Signal type
Stage temperature
Map data
Map data
Select raw data
Annotated data upload
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
Raw Data
Data upload
Pixel size For Data upload
Pixel size
Pixel units
Stage Tilt
Tilt angle (alpha axis)
Tilt angle (beta axis)
EMPIAR ID
Description of the raw data
Map data
Annotated data
Select raw data
Annotated data upload
Description of the annotated data
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
Raw Data
Data upload
Data upload
Pixel size
Pixel size
0.823
Pixel units
Å
Select the raw data type of SPA
Raw multiframe(raw movies)
Stage Tilt
Tilt angle (alpha axis)
Tilt angle (beta axis)
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Description of input data
Map data
Yes
Map data
Upload map data
Pixel size
Pixel size
0.823
Pixel units
Å
Select the map data type
Unsharpened map
Recommended contour level
Resolution at 0.143 FSC
Applied symmetry
Software for reconstruction
Other database id
Enter EMDB ID
Enter PDB ID
Description of map data
Raw Data
Other types of raw data
Data upload
Pixel size
Pixel size
Pixel units
Select the raw data type of SPA
Stage Tilt
Tilt angle (alpha axis)
Tilt angle (beta axis)
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Description of input data
Map data
Map data
Upload map data
Pixel size
Pixel size
Pixel units
Select the map data type
Recommended contour level
Resolution at 0.143 FSC
Helical parameter
Rise value of helical parameter
Twist value of helical parameter
Software for refinement
Other database id
Enter EMDB ID
Enter PDB ID
Description of map data
Raw Data
Upload raw tilt series / serial sectioning data
Annotated data
Select raw data
Pixel size
Pixel size
Pixel units
Tilt series
Minimum angle
Maximum angle
Angle imcrement
Excluded views
Or write manually
Tilt axis (image rotation)
Add fiducial markers
Description of tilt series/ serial sectioning data
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Upload tomogram
Pixel size
Pixel size
Pixel units
Software for reconstruction
Description of tomogram data
Upload labeled data
Pixel size
Pixel size
Pixel units
Software for segmentation
Description of labeled data
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
Raw Data
Upload averaged 3D map data
Pixel size
Pixel size
Pixel units
Recommended contour level
Resolution at 0.143 FSC
Applied symmetry
Software for refinement
Upload raw tilt series
Pixel size
Pixel size
Pixel units
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Description of input data
Annotated data
Select raw data
Tilt series
Minimum angle
Maximum angle
Angle imcrement
Excluded views
Or write manually
Tilt axis (image rotation)
Add fiducial markers
Description of tilt series
Upload tomogram
Pixel size
Pixel size
Pixel units
Software for reconstruction
Description of tomogram data
Upload subtomograms
Pixel size
Pixel size
Pixel units
Upload labeled data
Pixel size
Pixel size
Pixel units
Software for segmentation
Description of labeled data
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
typeOfInstrument
TEM
Category
Sample name
Scientific name
Natural source- Taxonomy ID (NCBI)
Sample Description
Category
Select raw data
Sample type
Sample name
Scientific name
Natural source- Taxonomy ID (NCBI)
Strain
Sample Description
Category
Select raw data
FoilHole_18251796_Data_18247291_22_20260819_053906_fractions.tiff
Sample type
Protein etc.
Protein
1 Protein name
TLR11
Number of copies
1
Recombinant protein sequence
Recombinant protein sequence
MLLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAAD PSEFLVDRSK NGLIHVPKDL SQKTTILNIS QNYISELWTS DILSLSKLRILIISHNRIQY LDISVFKFNQ ELEYLDLSHN KLVKISCHPT VNLKHLDLSF NAFDALPICK EFGNMSQLKF LGLSTTHLEK SSVLPIAHLNISKVLLVLGE TYGEKEDPEG LQDFNTESLH IVFPTNKEFH FILDVSVKTV ANLELSNIKC VLEDNKCSYF LSILAKLQTN PKLSNLTLNNIETTWNSFIR ILQLVWHTTV WYFSISNVKL QGQLDFRDFD YSGTSLKALS IHQVVSDVFG FPQSYIYEIF SNMNIKNFTV SGTRMVHMLCPSKISPFLHL DFSNNLLTDT VFENCGHLTE LETLILQMNQ LKELSKIAEM TTQMKSLQQL DISQNSVSYD EKKGDCSWTK SLLSLNMSSNILTDTIFRCL PPRIKVLDLH SNKIKSIPKQ VVKLEALQEL NVASNQLKSV PDGIFDRLTS LQKIWLHTNP WDCSCPRIDY LSRWLNKNSQKEQGSAKCSG SGKPVRSIIC PTGG
Description of recombinant protein sequence
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAADPSEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSKLRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQLKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSVKTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISNVKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPFLHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCSWTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVASNQLKSVPDGIFDRLTSLQKIWLHTNPWDCSCPRIDYLSRWLNKNSQKEQGSAKCSGSGKPVRSIICPTGG
Total molecular weight of all identical protomers
64 kDa
Natural source
Scientific name
synthetic construct
Natural source- Taxonomy ID (NCBI)
Strain
synthetic construct
Expression source
Natural source와 다름
Protein
Organism used for expression
Trichoplusia ni
NCBI Taxonomy ID of expression source
7111
Cell strain of expression source
2 Protein name
TLR2
Number of copies
1
Recombinant protein sequence
Recombinant protein sequence
MLLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAAD PESSNQASLS CDRNGICKGS SGSLNSIPSG LTEAVKSLDL SNNRITYISNSDLQRCVNLQ ALVLTSNGIN TIEEDSFSSL GSLEHLDLSY NYLSNLSSSW FKPLSSLTFL NLLGNPYKTL GETSLFSHLT KLQILRVGNMDTFTKIQRKD FAGLTFLEEL EIDASDLQSY EPKSLKSIQN VSHLILHMKQ HILLLEIFVD VTSSVECLEL RDTDLDTFHF SELSTGETNSLIKKFTFRNV KITDESLFQV MKLLNQISGL LELEFDDCTL NGVGNFRASD NDRVIDPGKV ETLTIRRLHI PRFYLFYDLS TLYSLTERVKRITVENSKVF LVPCLLSQHL KSLEYLDLSE NLMVEEYLKN SACEDAWPSL QTLILRQNHL ASLEKTGETL LTLKNLTNID ISKNSFHSMPETCQWPEKMK YLNLSSTRIH SVTGCIPKTL EILDVSNNNL NLFSLNLPQL KELYISRNKL MTLPDASLLP MLLVLKISRN QLKSVPDGIFDRLTSLQKIW LHTNPWDCSC PRIDYLSRWL NKNSQKEQGS AKCSGSGKPV RSIICPTGG
Description of recombinant protein sequence
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAADPESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDRVIDPGKVETLTIRRLHIPRFYLFYDLSTLYSLTERVKRITVENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACEDAWPSLQTLILRQNHLASLEKTGETLLTLKNLTNIDISKNSFHSMPETCQWPEKMKYLNLSSTRIHSVTGCIPKTLEILDVSNNNLNLFSLNLPQLKELYISRNKLMTLPDASLLPMLLVLKISRNQLKSVPDGIFDRLTSLQKIWLHTNPWDCSCPRIDYLSRWLNKNSQKEQGSAKCSGSGKPVRSIICPTGG
Total molecular weight of all identical protomers
67 kDa
Natural source
Scientific name
synthetic construct
Natural source- Taxonomy ID (NCBI)
Strain
synthetic construct
Expression source
Natural source와 다름
Protein
Organism used for expression
Trichoplusia ni
NCBI Taxonomy ID of expression source
7111
Cell strain of expression source
Ligand
Name of ligand
Description of ligand
Nucleic acid
Nucleic acid sequence
Description of nucleic acid
Natural source- Sample Description
Name
Scientific Name
Natural source- Taxonomy ID (NCBI)
Sample Description
Type of sample preparation method
Vitrification(for cryo-EM)
Sample preparation method information
Type of fixation
Fixation Infomation
Fixation description
Pre-staining solution formulation
Description of pre-staining procedure
Embedding resin
Resin mixture formulation
Ultra-thin section type
Thickness of section
Depth of serial section
Post-staining solution formulation
Description of post-staining method
Immuno-labeling
Immuno labeling object for fixation
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Fixation Infomation
Vitrification method
Equipment model name for Plunge freezing
Description of Plunge freezing
Equipment model name for high pressure freezing
Cryoprotectant for high pressure freezing
Description of high pressure freezing
Ultramicrotome Type
Equipment name for freeze substitution
Description of freeze substitution
Embedding resin
Resin mixture formulation
Ultra-thin section type
Thickness of section
Depth of serial section
Post-staining solution formulation
Description of post-staining method
Immuno-labeling
Immuno labeling
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sample preparation method information
Concentration of sample used
Sample concentration
Unit
Applied sample volume
Staining solution
Description of staining
Grid Manufacturer
Grid type
Glow discharge applied
Surface polarity
Surface charge
Atmosphere
Immuno-labeling
Immuno labeling object for fixation
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sample preparation method information
Vitrification method
Plunge freezing
Vitrification information
Concentration of sample used
Sample concentration
0.1
Unit
mg/mL
Applied sample volume
Plunge freezer manufacturer
Plunge freezer model
Grid Manufacturer
Grid type
Glow discharge applied
No
Surface polarity
Surface charge
Atmosphere
Description of vitrification
lipid monolayer
Immuno-labeling
No
Immuno labeling object for vitrification
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Vitrification information
Method name
Concentration of sample used
Sample concentration
Unit
Applied sample volume
Plunge freezer manufacturer
Plunge freezer model
Grid Manufacturer
Grid type
Glow discharge applied
Surface polarity
Surface charge
Atmosphere
Description of vitrification
Immuno-labeling
Immuno labeling object for vitrification
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sample preparation method information
Method name
Description of method
Immuno Labeling
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sputtering
Coat target
Discharge current
Deposition time
Description of sputtering
typeOfInstrument
TEM
typeOfInstrument
TEM
Site
Site of data collection
IMP, POSTECH
Site Address
Imaging Instrument - Manufacturer
Thermo Fisher Scientific(FEI)
Imaging Instrument - Model
Krios G4
Beam Source - Accelerating Voltage
300 kV
Imaging mode
Bright field
Defocus range
Min
Max
Gun type
Gun type
Thermonic emission type
Filed emission type
Related data
KBI
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