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전자현미경 데이터
FLuc-mRNA를 탑제한 SM-102 이온화가능지질 기반 지질나노입자(LNP)의 cryo-EM 이미지
  • Accession
    KIX10047796
  • Submission date
    2026-06-01
Project Detail
Dataset detail - Accession, 프로젝트의 영문 제목, 프로젝트의 국문 제목, 프로젝트의 영문 설명, 프로젝트의 국문 설명
Accession
 KAP242374
프로젝트의 영문 제목
Development of new high-performance multivalent mRNA nanocarriers
프로젝트의 국문 제목
신규 고성능 다가 mRNA 나노전달체 개발
프로젝트의 영문 설명
As a key enabling technology for mRNA vaccine development, this project aims to establish proprietary high-performance multivalent mRNA nanocarrier platforms with improved vaccine efficacy, safety, and stability, while ensuring freedom-to-operate through novel material design. To this end, a diverse library of synthetic- and biomaterial-based multivalent nanoparticles for mRNA delivery will be generated. Lead candidates will be selected through iterative optimization guided by in vitro and in vivo assessments of safety, delivery performance, and biological efficacy.
프로젝트의 국문 설명
mRNA 백신 개발의 핵심 요소기술로서, 독자적 물질설계에 의해 특허 회피가 가능하며 실용화를 목표로 백신 효능, 안전성, 안정성이 향상된 고성능 다가 mRNA 나노전달체 원천기술을 확보함. 이를 위해 본 연구에서는 합성 기반 및 바이오소재 기반 신개념 mRNA 전달용 다가 나노입자를 다수 제조, 세포 및 동물에의 안전성 및 효능 검증 feedback을 토대로 물질 최적화 과정을 거쳐 유망 물질을 도출하고자 함
메타데이터 정보
메타데이터 입력
장비 유형
TEM
Name
김윤경
Email address
ykim@kribb.re.kr
Role assignment
Principal investgator
Contributor
1 Contributor name
전성훈
Contributor email
jsh100@kbsi.re.kr
Contributor role assignment
Researcher
Contributor role notation
Sample preparation, Raw data collection, Image analysis, Computational image processing
2 Contributor name
전해근
Contributor email
jhg89@kribb.re.kr
Contributor role assignment
Researcher
Contributor role notation
Sample preparation
3 Contributor name
정혜연
Contributor email
seikotop@kribb.re.kr
Contributor role assignment
Researcher
Contributor role notation
Sample preparation
typeOfInstrument
TEM
Representative image data file
Title - English
Cryo-EM image of FLuc mRNA-loaded SM-102-based lipid nanoparticles (LNPs)
Title - Korean
FLuc-mRNA를 탑제한 SM-102 이온화가능지질 기반 지질나노입자(LNP)의 cryo-EM 이미지
Keywords
LNP, cryo-EM
Experimental method
Snapshot(standard imaging)
Raw Data
Data upload
Pixel size
Pixel size
Pixel units
Accelerating Voltage
Working distance
Emission current
Signal type
Stage temperature
Map data
Map data
Select raw data
Annotated data upload
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
Raw Data
Data upload
Pixel size For Data upload
Pixel size
Pixel units
Stage Tilt
Tilt angle (alpha axis)
Tilt angle (beta axis)
EMPIAR ID
Description of the raw data
Map data
No
Annotated data
Select raw data
Annotated data upload
Description of the annotated data
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
Raw Data
Data upload
Data upload
Pixel size
Pixel size
Pixel units
Select the raw data type of SPA
Stage Tilt
Tilt angle (alpha axis)
Tilt angle (beta axis)
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Description of input data
Map data
Map data
Upload map data
Pixel size
Pixel size
Pixel units
Select the map data type
Recommended contour level
Resolution at 0.143 FSC
Applied symmetry
Software for reconstruction
Other database id
Enter EMDB ID
Enter PDB ID
Description of map data
Raw Data
Other types of raw data
Data upload
Pixel size
Pixel size
Pixel units
Select the raw data type of SPA
Stage Tilt
Tilt angle (alpha axis)
Tilt angle (beta axis)
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Description of input data
Map data
Map data
Upload map data
Pixel size
Pixel size
Pixel units
Select the map data type
Recommended contour level
Resolution at 0.143 FSC
Helical parameter
Rise value of helical parameter
Twist value of helical parameter
Software for refinement
Other database id
Enter EMDB ID
Enter PDB ID
Description of map data
Raw Data
Upload raw tilt series / serial sectioning data
Annotated data
Select raw data
Pixel size
Pixel size
Pixel units
Tilt series
Minimum angle
Maximum angle
Angle imcrement
Excluded views
Or write manually
Tilt axis (image rotation)
Add fiducial markers
Description of tilt series/ serial sectioning data
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Upload tomogram
Pixel size
Pixel size
Pixel units
Software for reconstruction
Description of tomogram data
Upload labeled data
Pixel size
Pixel size
Pixel units
Software for segmentation
Description of labeled data
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
Raw Data
Upload averaged 3D map data
Pixel size
Pixel size
Pixel units
Recommended contour level
Resolution at 0.143 FSC
Applied symmetry
Software for refinement
Upload raw tilt series
Pixel size
Pixel size
Pixel units
EMPIAR ID
Web database deposition ID
Enter EMDB ID
Enter PDB ID
Description of input data
Annotated data
Select raw data
Tilt series
Minimum angle
Maximum angle
Angle imcrement
Excluded views
Or write manually
Tilt axis (image rotation)
Add fiducial markers
Description of tilt series
Upload tomogram
Pixel size
Pixel size
Pixel units
Software for reconstruction
Description of tomogram data
Upload subtomograms
Pixel size
Pixel size
Pixel units
Upload labeled data
Pixel size
Pixel size
Pixel units
Software for segmentation
Description of labeled data
Light micrograph for CLEM
Light micrograph data upload
Light micrograph - Manufacturer and model
Light source type
Objective Magnification
Optical section (Z)
Optical section (Z) thickness
Timelapse (T)
Timelapse (T) fps
Information for channels and target molecules
Data type
Process information
Image format
Image analysis program
Description of the light micrograph for CLEM
typeOfInstrument
TEM
Category
Sample name
Scientific name
Natural source- Taxonomy ID (NCBI)
Sample Description
Category
Select raw data
Sample type
Sample name
Scientific name
Natural source- Taxonomy ID (NCBI)
Strain
Sample Description
Category
Select raw data
FoilHole_16389121_Data_16386663_16386665_20241017_000427.jpg,FoilHole_16389121_Data_16390680_16390682_20241017_000448.jpg,FoilHole_16389129_Data_16386663_16386665_20241017_001353.jpg,FoilHole_16389130_Data_16386663_16386665_20241017_001504.jpg,FoilHole_16389133_Data_16390680_16390682_20241017_001638.jpg,FoilHole_16389136_Data_16386663_16386665_20241017_002007.jpg,FoilHole_16389136_Data_16390680_16390682_20241017_002030.jpg,FoilHole_16389137_Data_16390680_16390682_20241017_002138.jpg,FoilHole_16389138_Data_16386663_16386665_20241017_002224.jpg,FoilHole_16389138_Data_16390680_16390682_20241017_002247.jpg,FoilHole_16389139_Data_16386663_16386665_20241017_002357.jpg,FoilHole_16389139_Data_16390680_16390682_20241017_002420.jpg,FoilHole_16389145_Data_16390680_16390682_20241017_002706.jpg,FoilHole_16389156_Data_16386663_16386665_20241017_003411.jpg,FoilHole_16389157_Data_16386663_16386665_20241017_003543.jpg,FoilHole_16389157_Data_16390680_16390682_20241017_003605.jpg,FoilHole_16389166_Data_16386663_16386665_20241017_004325.jpg,FoilHole_16389170_Data_16386663_16386665_20241017_004701.jpg,FoilHole_16389170_Data_16390680_16390682_20241017_004724.jpg,FoilHole_16389171_Data_16386663_16386665_20241017_004813.jpg,FoilHole_16389173_Data_16390680_16390682_20241017_005123.jpg,FoilHole_16389174_Data_16386663_16386665_20241017_005212.jpg,FoilHole_16389174_Data_16390680_16390682_20241017_005235.jpg,FoilHole_16389175_Data_16390680_16390682_20241017_005346.jpg,FoilHole_16389176_Data_16386663_16386665_20241017_005435.jpg,FoilHole_16389181_Data_16386663_16386665_20241017_010009.jpg,FoilHole_16389183_Data_16390680_16390682_20241017_010255.jpg,FoilHole_16389186_Data_16390680_16390682_20241017_010541.jpg,FoilHole_16389191_Data_16386663_16386665_20241017_011033.jpg
Sample type
others(peptide,exosome, etc.)
Protein
Protein name
Number of copies
Recombinant protein sequence
Recombinant protein sequence
Description of recombinant protein sequence
Total molecular weight of all identical protomers
Natural source
Scientific name
Natural source- Taxonomy ID (NCBI)
Strain
Expression source
Protein
Organism used for expression
NCBI Taxonomy ID of expression source
Cell strain of expression source
Ligand
Name of ligand
Description of ligand
Nucleic acid
Nucleic acid sequence
Description of nucleic acid
Natural source- Sample Description
Name
Scientific Name
Lipid nanoparticle (LNP)
Natural source- Taxonomy ID (NCBI)
Sample Description
An SM-102 ionizable lipid-based lipid nanoparticle (LNP) encapsulating FLuc mRNA
Type of sample preparation method
Vitrification(for cryo-EM)
Sample preparation method information
Type of fixation
Fixation Infomation
Fixation description
Pre-staining solution formulation
Description of pre-staining procedure
Embedding resin
Resin mixture formulation
Ultra-thin section type
Thickness of section
Depth of serial section
Post-staining solution formulation
Description of post-staining method
Immuno-labeling
Immuno labeling object for fixation
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Fixation Infomation
Vitrification method
Equipment model name for Plunge freezing
Description of Plunge freezing
Equipment model name for high pressure freezing
Cryoprotectant for high pressure freezing
Description of high pressure freezing
Ultramicrotome Type
Equipment name for freeze substitution
Description of freeze substitution
Embedding resin
Resin mixture formulation
Ultra-thin section type
Thickness of section
Depth of serial section
Post-staining solution formulation
Description of post-staining method
Immuno-labeling
Immuno labeling
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sample preparation method information
Concentration of sample used
Sample concentration
Unit
Applied sample volume
Staining solution
Description of staining
Grid Manufacturer
Grid type
Glow discharge applied
Surface polarity
Surface charge
Atmosphere
Immuno-labeling
Immuno labeling object for fixation
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sample preparation method information
Vitrification method
Plunge freezing
Vitrification information
Concentration of sample used
Sample concentration
6.4
Unit
Others
Applied sample volume
3.5 μL
Plunge freezer manufacturer
Thermo Fisher Scientific Inc.
Plunge freezer model
Vitrobot Mark IV
Grid Manufacturer
Electron Microscopy Sciences
Grid type
LC300-Cu
Glow discharge applied
Yes
Surface polarity
Hydrophillic
Surface charge
Negative
Atmosphere
Air
Description of vitrification
Immuno-labeling
No
Immuno labeling object for vitrification
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Vitrification information
Method name
Concentration of sample used
Sample concentration
Unit
Applied sample volume
Plunge freezer manufacturer
Plunge freezer model
Grid Manufacturer
Grid type
Glow discharge applied
Surface polarity
Surface charge
Atmosphere
Description of vitrification
Immuno-labeling
Immuno labeling object for vitrification
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sample preparation method information
Method name
Description of method
Immuno Labeling
Immuno-labeling method
Experiment condition
Labeling method
1st Antibody
Pimary antibody
Conjugated material of Primary antibody
Diameter of conjugated material
2nd Antibody
Secondary antibody
Conjugated material of Secondary antibody
Diameter of conjugated material
Description of immunostaining procedure
Sputtering
Coat target
Discharge current
Deposition time
Description of sputtering
typeOfInstrument
TEM
typeOfInstrument
TEM
Site
Site of data collection
Korea Basic Science Institute (KBSI)
Site Address
162 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea
Imaging Instrument - Manufacturer
Thermo Fisher Scientific(FEI)
Imaging Instrument - Model
Talos Arctica G2
Beam Source - Accelerating Voltage
200 kV
Imaging mode
Bright field
Defocus range
Min
Max
Gun type
Gun type
Thermonic emission type
Filed emission type
Related data
KBI
  • 전자현미경 데이터
    1

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