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Total 50,000 Hep3B cells were washed two times with 50ul cold PBS, and resuspended with 50ul cold lysis buffer10 mM Tris-Cl, pH7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40. After centrifugation at 2,000 rpm for 10 min at 4?? nuclei were incubated in fresh 25ul TD Buffer 10mM Tris-HCl pH8.0, 5mM MgCl2, 10% dimethylformamide with 2ul Tn5 Transposase and 23ul DW at 37degree Celsius for 30min. QIAquick PCR purification kitQiagen; cat. no. 28106 was used to purify DNA fragments. We used HiFi HotStart ReadyMix KAPA; KK2601 for library amplification following provided manual except number of PCR cycle: we performed 15 cycle for PCR amplification. The amplified library was purified with a QIAquick PCR purification kit Qiagen; cat. no. 28106 and sequenced using a NovaSeq2500 system150bp with the paired method.