Run
KAR21040520
Experiment Title
Illumina HiSeq 4000 paired Sequencing of KAS20000262
(PRJDB19217)
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Run Metadata
Primary
(1 cases)
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file type
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location
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file name
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bytes
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total sequences (bp)
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sequence lengh (bp) | Release date | Access type |
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| fastq | KBDS-KRA | 71,323,505,066 71,323,505,066 | 2021-12-03 | PUBLIC |
library_construction_protocol
In-situ Hi-C was performed as previously described Rao et al., 2014. Briefly: T47D cells were crosslinked with 1% formaldehyde; nuclei were isolated; chromatin was digested with MboI R0147, NEB; 5??ends were filled by incorporation of biotinylated-dCTP 19524-016, Life Technologies; proximity ligation, reverse-crosslinking, and DNA shearing were performed; biotinylated junctions were isolated with streptavidin beads 65601, Life Technologies; and DNA libraries were prepared. Each Hi-C library was amplified for six cycles and then subjected to deep sequencing on a Hiseq4000 Illumina platform. The libraries were generated from two sets of biological replicates.
Analysis
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Reference
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| reference type | RefSeq accession ID | File name |
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| No Run data. | ||