Project goal ○ Whole genome and transcriptome analyses of the starry flounder (Platichthys stellatus) - Genome sequencing of individual marine animals using the PacBio-Sequel and the HiSeq Paired-end methods - Transcriptome sequencing using the HiSeq Paired-end and the IsoSeq-Sequel sequencing methods, and DEG analysis using templates prepared from individual marine animals at varying time points after artificial infection or stimulation ○ Acquisition and analysis of useful genes - Selection of 15 or more useful genes from each marine animal - Expression analysis of 8 or more genes from the selected useful gene pool - Production of three or more of the selected useful gene products by recombinant expression systems or peptide synthesis and ligation to identify their functions Project contents ○ Production of whole-genome data from the starry flounder - Genomic DNA extraction from a starry flounder population - Approximately 49 Gb of data production using the PacBio-Sequel (>20 kb) sequencing - Approximately 28 Gb of data production using the HiSeq Paired-end (2 × 101 bp) sequencing ○ Production of the starry flounder transcriptome data - 36 samples isolated and prepared at different time periods after artificial infection or stimulation for differential gene expression analysis - Production of 5 Gb of data per sample using the HiSeq Paired-end (2 × 101 bp) sequencing - Production of 5 Gb of data per cell (1 SMRT cell) using the IsoSeq-Sequel sequencing "Expected efficacy and applications ○ Providing technology to aid academic and industrial use of major aquaculture and aquatic biological resources through the completion of aquatic standard dielectric and establishment of a specialized genomic data platform for aquatic organisms ○ Accelerating research on aquacultured species by establishing economical methods to identify useful genes and gene markers ○ Creation of new industries by developing bio materials using the genomic big data "Expectation effectiveness and application ○ Providing technology for academic and industrial use of major aquaculture and aquatic biological resources through completion of aquatic standard dielectric and establishment of aquatic specialized genomic data platform ○ Accelerate research on aquaculture breeds by searching for useful economic and useful genes and gene markers ○ Create new industries by developing bio materials using genome big data
