Bottom-up proteomic profiling of extracellular vesicle (EV) extracted from human plasma by four isolation methods - ultracentrifugation, size exclusion chromatography, density gradient ultracentrifugation, and hydrogel-based isolation. Samples were prepared according to modified S-trap protocol with concentration of SDS adjusted to 2% for lysis buffer followed by trypsin digestion. LC-MS/MS analysis was performed with Thermo Scientific Ultimate 3000 and Thermo Q-Exactive HF-X connected by Thermo ES903 50cm column. Proteins were identified and quantified in label-free quantitation mode by Proteome Discoverer v2.4
