scDB: Study

Study

Study Single cell lncRNA atlas of mouse tissues identifies lncRNA-mediated cell type-specific gene regulatory network

1. Library construction for analysis of coding and non-coding transcriptome at the single cell level of mouse. 2. With combining conventional single-cell RNA sequencing (scRNA-seq) and targeted sequencing, investigation of lncRNAs that difficult to detect conventional scRAN-seq method. 3. Centered on cell specific lncRNAs and TFs, determination the biological functions of lncRNA by constructing gene regulatory networks(GRNs). 4. Based on GRNs and lncRNAs expression levels, establishment of web-server as a resource for lncRNA research at the single cell levels.

Study Information

Accession

SCS2100481

Date and Organization


Processed date	2022-07-28
Oranization	Gwangju Institute of Science and Technology

Publication


PubMed ID of publication
DOI of publication

Conditions

Species


Scientific Name	Mus musculus
Taxonomy ID	10090


Tissue	Bodily fluid, Heart, Intestine, Kidney, Liver, Lung, Non-organ sample, Thymus	Treatment	Cells were harvested by trypsinization for NIH/3T3 and scraping for RAW 264.7. Harvested cells were washed and resuspended in 0.04% BSA in 1X DPBS., Cells were harvested by trypsinization for NIH/3T3 and scraping for RAW 264.7. Harvested cells were washed and resuspended in 0.04% BSA in 2X DPBS., Cells were harvested by trypsinization for NIH/3T3 and scraping for RAW 264.7. Harvested cells were washed and resuspended in 0.04% BSA in 3X DPBS., Cells were harvested by trypsinization for NIH/3T3 and scraping for RAW 264.7. Harvested cells were washed and resuspended in 0.04% BSA in 4X DPBS., Organs were isolated after Co10 euthanasia, Organs were isolated after Co11 euthanasia, Organs were isolated after Co12 euthanasia, Organs were isolated after Co13 euthanasia, Organs were isolated after Co14 euthanasia, Organs were isolated after Co15 euthanasia, Organs were isolated after Co16 euthanasia, Organs were isolated after Co17 euthanasia, Organs were isolated after Co18 euthanasia, Organs were isolated after Co19 euthanasia, Organs were isolated after Co2 euthanasia, Organs were isolated after Co20 euthanasia, Organs were isolated after Co21 euthanasia, Organs were isolated after Co22 euthanasia, Organs were isolated after Co23 euthanasia, Organs were isolated after Co24 euthanasia, Organs were isolated after Co3 euthanasia, Organs were isolated after Co4 euthanasia, Organs were isolated after Co5 euthanasia, Organs were isolated after Co6 euthanasia, Organs were isolated after Co7 euthanasia, Organs were isolated after Co8 euthanasia, Organs were isolated after Co9 euthanasia
Sex	Male	Cell Line	CRL-1658, TIB-71
Age	8 weeks	Cell Type	Fibroblast, Macrophage
Development Stage	Adult	Cell Subtype
Disease	-	Isolate	-
Disease Stage	-	Genotype	wild type
Helath State	-

Associated K-BDS/scDB


BioProject	KAP210048	scDB Study	N/A
BioSample	All samples [27]	scDB Data	All Data [27]
KRA	All experiments [27]

Analysis pipeline(v2025.12)

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