Data
Data
kidney_pre_capture
Data Information
|
Accession
|
SCD21087650 | ||||
|---|---|---|---|---|---|
|
Date and Organization
|
|
||||
|
Library Construction
|
Library preparation for single-cell RNA-seq was followed according to the Chromium Single Cell 3??Reagent Kits User Guide v3.1, 10X Genomics. Single-cell suspensions were filtered through a 40um Flowmi cell strainer Bel-Art, counted using a CountessII automated cell counter ThermoFisher, and then loaded onto a microfluidic chip. In the Chromium Controller, cells were separated into Gel Beads-in-emulsion GEMs where poly adenylated RNAs in individual cells were tagged with a UMI unique molecular identifier and cell barcode. As soon as GEM generation was finished, reverse transcription was performed to produce barcoded cDNAs. cDNAs were amplified through PCR and amplified cDNAs were used for sequencing library construction. Briefly, cDNA amplicons were enzymatically fragmented, end-repaired, dA-tailed, and ligated with a sequencing adaptor. The final sequencing libraries were generated by sample index PCR and sequenced using a HiSeq2500 Illumina. pre_capture: conventional single cell RNA sequencing |
Conditions
|
Species
|
|
|---|
Tissue |
Kidney | Treatment |
Organs were isolated after Co2 euthanasia |
|---|---|---|---|
Sex |
Male | Cell Line |
- |
Age |
8 weeks | Cell Type |
- |
Development Stage |
Adult | Cell Subtype |
- |
Disease |
- | Isolate |
- |
Disease Stage |
- | Genotype |
wild type |
Helath State |
- | ||
Associated K-BDS/scDB
|
BioProject
|
KAP210048 |
scDB Study
|
SCS2100481 |
|---|---|---|---|
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BioSample
|
All samples [1] |
scDB Data
|
All Data [1] |
|
KRA
|
All experiments [1] | ||