Synthetic Construct
Synthetic construct for Homo Sapiens GPX4 using pET-28a(+), sequence
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AccessionKSYN010000000284
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Submission date2026-09-28
Project Detail
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BioProject
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KAP242604 |
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Project title
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Industrialization platform for structure-guided drug discovery
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Project description
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Structural Studies on GPX4 and Mesh1 Proteins and Structure-Based Drug Discovery (SBDD)
Establish a structural understanding for discovering ferroptosis-modulating drugs by integrating structural studies on GPX4 and Mesh1 complexes.
- Identification of target-specific binders via screening.
- Structural determination of target protein–ligand complexes.
- Elucidation of the target mechanism of action based on complex structures.
- Generation of lead compounds through medicinal chemistry.
- Evaluation of target-binding affinity and determination of binding modes for lead compounds.
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Name of submitter
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Lee Hyeonjun
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DataSet
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Name of submitter's organization
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Korea University
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Department of submitter
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College of Pharmacy
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Country of submitter
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South Korea
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Street address
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2511 Sejong-ro, Jochiwon-eup
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City
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Sejong-si
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State / Province / Region
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Sejong-si
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Zip Code (Postal Code)
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30019
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Contact person information
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Publication status
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Unpublished
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Publication
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Reference title
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Discovery of GPX4 inhibitor
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Confirm update of existing submission
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No
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Existing KNA Accession ID
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Sequence Authors
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Sample processing protocol
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Data processing protocol
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Supplementary information
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Release Protocol?
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No
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Topology
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Circular
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Feature Information
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Sequence source (Backbone)
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pET-28a(+)
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Sequence source Accession
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Target
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Homo Sapiens GPX4
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Name
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pET-28a(+)-His-SUMO-GPX4(C2S, C10A, C37S, U46C, C66R, C75S, C107E, C148V)
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Purpose
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Expression of human GPX4 mutant protein
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Description
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Plasmid created by inserting Homo sapiens GPX4(C2S, C10A, C37S, U46C, C66R, C75S, C107E, C148V) into a pET-28a(+) vector for GPX4(U46C) protein expression
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Annotation file format
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None
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Total length
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6097
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Total contigs
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1
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Scaffolds or chromosomes Total length
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Scaffolds or chromosomes Total contigs
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| File Name | Size | Type | Published | Download | |
|---|---|---|---|---|---|
| Synthetic construct for Homo Sapiens GPX4 using pET-28a(+), sequence.fasta | 6,295 6,295 | fasta | 2026-09-29 |