합성 유전자 서열
Synthetic construct for Custom gene of interest(GOI, empty backbone) using Backbone, sequence
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AccessionKSYN010000000042
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Submission date2025-10-24
Project Detail
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BioProject
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KAP241683 |
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프로젝트의 영문 제목
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Establishment and Support of a Bio-commercialization Innovation Platform
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프로젝트의 영문 설명
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◦ This center supports the bioindustry by providing industrial equipment for key process development, prototype production, academic-industry collaboration, and workforce training.
◦ We develop core biopharmaceutical technologies such as expression systems, suspension cultures, purification, and analysis to strengthen commercialization capabilities.
◦ We conduct process development for biomaterials through strain engineering, fermentation, downstream processing, and scale-up technologies to reach global standards.
◦ By supporting process development and pilot production, we help bio companies shorten their time to market.
◦ We promote research collaboration and support underdeveloped technologies by sharing infrastructure and research outcomes.
◦ We also offer equipment access and practical training to expand infrastructure and support industrial workforce development.
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등록자의 영문 이름
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Lee Eun Gyo
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DataSet
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Name of submitter's organization
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Korea Research Institute of Bioscience and Biotechnology(KRIBB)
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Department of submitter
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BioProcess Engineering Center
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Country of submitter
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South Korea
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Street address
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125 Gwahak-ro
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City
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Yuseong-gu
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State / Province / Region
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Daejeon
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Zip Code (Postal Code)
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34141
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Contact person information
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Publication status
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Unpublished
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Publication
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Reference title
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Confirm update of existing submission
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No
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Existing KNA Accession ID
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Sequence Authors
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Sample processing protocol
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Data processing protocol
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Supplementary information
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Clone the GOI into the MCS using the NheI/XhoI restriction sites to achieve CMV-driven expression in mammalian cells.
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Release Protocol?
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No
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Topology
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Feature Information
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Sequence source (Backbone)
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Backbone
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Sequence source Accession
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Target
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Custom gene of interest(GOI, empty backbone)
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Name
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pWZ KRIBB
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Purpose
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Insert-ready mammalian expression vector
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Description
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A custom-designed mammalian expression backbone containing the CMV promoter for strong constitutive transgene expression. The vector includes WPRE to enhance mRNA stability and expression efficiency and a BleoR cassette for Zeocin resistance in both mammalian and bacterial cells. Suitable for insertion of any gene of interest through MCS.
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Annotation file format
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None
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Total length
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6267
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Total contigs
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1
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Scaffolds or chromosomes Total length
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Scaffolds or chromosomes Total contigs
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| File Name | Size | Type | Published | Download | |
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| pWZKRIBB.gb | 13,251 13,251 | gb | 2025-11-04 | ||
| Synthetic construct for Custom gene of interest(GOI, empty backbone) using Backbone, sequence.fasta | 6,488 6,488 | fasta | 2025-11-04 |