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Sequencing-based functional genomics data
KSE102968
Title
Single-cell transcriptomics reveals prolactin-associated molecular stratification and remission risk in somatotroph tumors
Study
Study Fields - Title, Summary, Experimental design, Experimental type, Organism, Source name, Contributor(s) Citation, Submission type
Title
 Single-cell transcriptomics reveals prolactin-associated molecular stratification and remission risk in somatotroph tumors
Summary
We performed single-cell RNA sequencing of surgically resected growth hormone (GH)-secreting somatotroph pituitary neuroendocrine tumors (PitNETs) from 12 patients with acromegaly to characterize tumor-intrinsic transcriptional heterogeneity and patient-level molecular stratification. The study identified structured somatotroph transcriptional states and a prolactin (PRL)-associated transcriptional stratification. Processed 10x Genomics feature-barcode count matrices are provided for each tumor.
Experimental design
population based design
Experimental type
RNA-seq of total RNA
Organism
 Homo sapiens
Contributor(s)
 Oh,H.;;Shim,J.;Oh,H.;Kim,E.
Submission type
 Partial
Protocols
Protocols - Accession, Type, Description
Accession
Type
Description
KSP10029834 Sample collection protocol
Fresh somatotroph pituitary neuroendocrine tumor specimens were obtained from patients with acromegaly during endoscopic transsphenoidal surgery. Fresh tumor specimens were immediately processed for single-cell dissociation.
KSP10029835 Nucleic acid extraction protocol
Fresh tumor tissues were dissociated into single-cell suspensions
KSP10029836 Nucleic acid library construction protocol
library was prepared using 10x Genomics Chromium Next GEM Single Cell 3' kit
KSP10029837 Nucleic acid sequencing protocol
Prepared 10X library went under the illumina sequencing platform
KSP10029838 Normalization data transformation protocol
Sequencing data were processed using Cell Ranger v8.0.1 with the 10x Genomics human GRCh38 reference (refdata-gex-GRCh38-2024-A) for alignment, barcode assignment, and gene counting.
Experimental characteristics
Experimental characteristics Fields - Library strategy, Library source, Library selection, Instrument model
Library strategy
 RNA-Seq
Library source
 TRANSCRIPTOMIC SINGLE CELL
Library selection
 Oligo-dT
Instrument model
 Illumina NovaSeq 6000
Detailed Experiment information
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Showing 1-12 of 12
Validations details list
Accession ID BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others BioSample accession ID Sample name Organism NCBI taxonomy ID sampleGroup Complete Data type Reference data ID type Reference data ID type Reference data ID type Reference data ID Library name Sample detail ChIP antibody Material type Factor value Protocols Description Platform ID Cell enrichment End bias Amplification method Channels and image type title Library strategy Library source Library selection Library layout Platform Instrument model Library construction protocol Library preparation kit manufacturer Library preparation kit Single cell isolation Single cell entity Single cell isolation protocol Tissue section preservation Section thickness Section preparation Staining method Staining protocol Magnification Capture area Observation protocol Input molecule Primer Spike-ins cDNA read UMI barcode read Cell barcode read Spatial barcode read Sample multiplexing method Sample multiplexing protocol Multiplexing identifiers per sample Sample barcode read Count matrix / Raw counts Browser extensible data(.bed) NarrowPeak (.narrowPeak) BroadPeak (.broadPeak) BedGraph (.bedgraph) WIG (.wig) BIGWIG (.bw) Count matrix - Normalize Cell barcode matrix Feature information matrix Spatial information Alignment files Image (.jpg, .tiff) Reference genome type Reference genome info Processed files Others
KSX10001090 KAS24222114 Acro1 Homo sapiens 9606 Single-cell sequencing None Acro1_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro1 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro1matrix.mtx.gz Acro1barcodes.tsv.gz Acro1features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001091 KAS24222115 Acro2 Homo sapiens 9606 Single-cell sequencing None Acro2_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro2 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro2matrix.mtx.gz Acro2barcodes.tsv.gz Acro2features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001092 KAS24222116 Acro3 Homo sapiens 9606 Single-cell sequencing None Acro3_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro3 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro3matrix.mtx.gz Acro3barcodes.tsv.gz Acro3features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001093 KAS24222117 Acro4 Homo sapiens 9606 Single-cell sequencing None Acro4_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro4 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro4matrix.mtx.gz Acro4barcodes.tsv.gz Acro4features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001094 KAS24222118 Acro5 Homo sapiens 9606 Single-cell sequencing None Acro5_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro5 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro5matrix.mtx.gz Acro5barcodes.tsv.gz Acro5features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001095 KAS24222119 Acro6 Homo sapiens 9606 Single-cell sequencing None Acro6_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro6 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro6matrix.mtx.gz Acro6barcodes.tsv.gz Acro6features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001096 KAS24222120 Acro7 Homo sapiens 9606 Single-cell sequencing None Acro7_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro7 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro7matrix.mtx.gz Acro7barcodes.tsv.gz Acro7features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001097 KAS24222121 Acro8 Homo sapiens 9606 Single-cell sequencing None Acro8_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro8 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro8matrix.mtx.gz Acro8barcodes.tsv.gz Acro8features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001098 KAS24222122 Acro9 Homo sapiens 9606 Single-cell sequencing None Acro9_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro9 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro9matrix.mtx.gz Acro9barcodes.tsv.gz Acro9features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001099 KAS24222123 Acro10 Homo sapiens 9606 Single-cell sequencing None Acro10_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro10 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro10matrix.mtx.gz Acro10barcodes.tsv.gz Acro10features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001100 KAS24222124 Acro11 Homo sapiens 9606 Single-cell sequencing None Acro11_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro11 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro11matrix.mtx.gz Acro11barcodes.tsv.gz Acro11features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
KSX10001101 KAS24222125 Acro12 Homo sapiens 9606 Single-cell sequencing None Acro12_TRANSCRIPTOMIC_SINGLE_CELL Freshly resected GH-secreting somatotroph pituitary neuroendocrine tumor tissue from a patient with acromegaly polyA RNA Disease: Acromegaly KSP10029834, KSP10029835, KSP10029836, KSP10029837, KSP10029838 Processed single-cell RNA-seq data from a freshly dissociated somatotroph pituitary neuroendocrine tumor from a patient with acromegaly. Illumina NovaSeq 6000 paired-end Sequencing of Acro12 RNA-Seq TRANSCRIPTOMIC SINGLE CELL Oligo-dT paired ILLUMINA Illumina NovaSeq 6000 Single-cell 3' gene expression libraries were prepared from freshly dissociated tumor cells using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics) according to the manufacturer's instructions. 10x Genomics 10x 3’ v2 droplet-based cell isolation whole cell Fresh tumor specimens were immediately processed for single-cell dissociation to minimize transcriptional artifacts. Tumor tissues were enzymatically dissociated into single-cell suspensions using standard protocols. Cell viability was assessed prior to library preparation. None none Acro12matrix.mtx.gz Acro12barcodes.tsv.gz Acro12features.tsv.gz RefSeq GRCh38 (10x Genomics refdata-gex-GRCh38-2024-A)
1현재 페이지
Accession
Accession - RUNs, BIOSAMPLEs
BioSamples
BioProject
 KAP242524
Files
KEA file 목록
File Name Sample IDS Size Format File type Release date Download
Acro1barcodes.tsv.gz
KAS24222114
6,900,909 6,900,909 gz
Cell barcode matrix
 2026-09-03
Acro1features.tsv.gz
KAS24222114
491,230 491,230 gz
Feature information matrix
 2026-09-03
Acro1matrix.mtx.gz
KAS24222114
175,831,966 175,831,966 gz
Count matrix / Raw counts
 2026-09-03
Acro2barcodes.tsv.gz
KAS24222115
7,341,887 7,341,887 gz
Cell barcode matrix
 2026-09-03
Acro2features.tsv.gz
KAS24222115
491,230 491,230 gz
Feature information matrix
 2026-09-03
Acro2matrix.mtx.gz
KAS24222115
174,326,805 174,326,805 gz
Count matrix / Raw counts
 2026-09-03
Acro3barcodes.tsv.gz
KAS24222116
10,492,774 10,492,774 gz
Cell barcode matrix
 2026-09-03
Acro3features.tsv.gz
KAS24222116
491,230 491,230 gz
Feature information matrix
 2026-09-03
Acro3matrix.mtx.gz
KAS24222116
188,280,648 188,280,648 gz
Count matrix / Raw counts
 2026-09-03
Acro4barcodes.tsv.gz
KAS24222117
6,242,030 6,242,030 gz
Cell barcode matrix
 2026-09-03
Acro4features.tsv.gz
KAS24222117
294,478 294,478 gz
Feature information matrix
 2026-09-03
Acro4matrix.mtx.gz
KAS24222117
169,665,034 169,665,034 gz
Count matrix / Raw counts
 2026-09-03
Acro5barcodes.tsv.gz
KAS24222118
6,045,121 6,045,121 gz
Cell barcode matrix
 2026-09-03
Acro5features.tsv.gz
KAS24222118
294,478 294,478 gz
Feature information matrix
 2026-09-03
Acro5matrix.mtx.gz
KAS24222118
241,868,917 241,868,917 gz
Count matrix / Raw counts
 2026-09-03
Acro6barcodes.tsv.gz
KAS24222119
4,745,007 4,745,007 gz
Cell barcode matrix
 2026-09-03
Acro6features.tsv.gz
KAS24222119
301,289 301,289 gz
Feature information matrix
 2026-09-03
Acro6matrix.mtx.gz
KAS24222119
192,726,448 192,726,448 gz
Count matrix / Raw counts
 2026-09-03
Acro7barcodes.tsv.gz
KAS24222120
5,536,497 5,536,497 gz
Cell barcode matrix
 2026-09-03
Acro7features.tsv.gz
KAS24222120
301,289 301,289 gz
Feature information matrix
 2026-09-03
Acro7matrix.mtx.gz
KAS24222120
267,584,828 267,584,828 gz
Count matrix / Raw counts
 2026-09-03
Acro8barcodes.tsv.gz
KAS24222121
5,704,514 5,704,514 gz
Cell barcode matrix
 2026-09-03
Acro8features.tsv.gz
KAS24222121
301,289 301,289 gz
Feature information matrix
 2026-09-03
Acro8matrix.mtx.gz
KAS24222121
244,913,748 244,913,748 gz
Count matrix / Raw counts
 2026-09-03
Acro9barcodes.tsv.gz
KAS24222122
6,920,180 6,920,180 gz
Cell barcode matrix
 2026-09-03
1현재 페이지2