Array-based functional genomics data
KSE102923
Title
Expression data from Ad-Vaspin infected chondrocytes
Study
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Title
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Expression data from Ad-Vaspin infected chondrocytes |
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Summary
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Adenovirus Vaspin infection in mouse chondrocytes. After isiolation of RNA, metrix microarrays_St2 was perfromed
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Experimental design
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compound treatment design
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Experimental type
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Transcription profiling by array
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Organism
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Mus musculus molossinus |
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Source name
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Articular chondrocytes from mouse with Primary culture were incubated for 5 days |
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Contributor(s)
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- |
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Submission type
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Complete |
Protocols
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Accession
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Type
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Description
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|---|---|---|
| KSP10028340 | Nucleic acid labeling protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix
protocol from 6 ㎍ total RNA (Expression Analysis Technical Manual, 2001,
Affymetrix)
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| KSP10028341 | Nucleic acid hybridization to array protocol |
0 ㎍ of cRNA were hybridized for 16 hr at 45°C on
GeneChip mouse micro Array. GeneChips were washed and stained
in the Affymetrix Fluidics Station 400
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| KSP10028342 | Array scanning and feature extraction protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
G2500A.
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| KSP10028338 | Sample collection protocol |
missing
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| KSP10028339 | Nucleic acid extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's
instructions.
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| KSP10028343 | Normalization data transformation protocol |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using
Affymetrix default analysis settings and global scaling as normalization method.
The trimmed mean target intensity of each array was arbitrarily set to 100.
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Detailed Experiment information
Toggle table
Accession
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BioSamples
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BioProject
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KAP240857 |
| File Name | Sample IDS | Size | Format | File type | Release date | Download | |
|---|---|---|---|---|---|---|---|
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Adaw2000.CEL
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26,332,519 26,332,519 | CEL |
Raw data file
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2024-09-30 | |||
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data11.txt
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1,720,039 1,720,039 | txt |
Abundance matrix file
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2024-09-30 |