KCODE: Text Search

KCP10001203

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

SVH07

ChEMBL

CHEMBL1315992

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

97

보고되지 않음

0

KCP10001204

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

21

ChEMBL

CHEMBL4177074

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

91

보고되지 않음

0

KCP10001205

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

22

ChEMBL

CHEMBL4166446

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

82

보고되지 않음

0

KCP10001206

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

23

ChEMBL

CHEMBL4173703

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

54

보고되지 않음

0

KCP10001207

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

24

ChEMBL

CHEMBL4162998

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

99

보고되지 않음

0

KCP10001208

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

25

ChEMBL

CHEMBL4163506

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

68

보고되지 않음

0

KCP10001209

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

26

ChEMBL

CHEMBL4174207

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

91

보고되지 않음

0

KCP10001210

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

27

ChEMBL

CHEMBL4166954

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

92

보고되지 않음

0

KCP10001211

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

28

ChEMBL

CHEMBL4159052

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

91

보고되지 않음

0

KCP10001212

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

29

ChEMBL

CHEMBL4175256

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

29

보고되지 않음

0

KCP10001213

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

30

ChEMBL

CHEMBL4167387

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

82

보고되지 않음

0

KCP10001214

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

31

ChEMBL

CHEMBL4160135

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

99

보고되지 않음

0

KCP10001215

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

32

ChEMBL

CHEMBL4170783

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

99

보고되지 않음

0

KCP10001216

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

33

ChEMBL

CHEMBL4168455

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

96

보고되지 않음

0

KCP10001217

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

34

ChEMBL

CHEMBL4160556

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

99

보고되지 않음

0

KCP10001218

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

35

ChEMBL

CHEMBL4170112

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

73

보고되지 않음

0

KCP10001219

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

36

ChEMBL

CHEMBL4159495

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

99

보고되지 않음

0

KCP10001220

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

37

ChEMBL

CHEMBL4173569

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

98

보고되지 않음

0

KCP10001221

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

38

ChEMBL

CHEMBL4162857

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

87

보고되지 않음

0

KCP10001222

KAP241919

Chemical Data Quality Management and Utilization

Verified the appropriateness of Target Product Profile (TPP) indicators for lead and candidate compound data to be registered in the compound data profiling section of the Korea BioData Station (K‑BDS) by gathering input from experts across various domestic fields. We also evaluated data‑quality management compliance and reviewed registration forms and indicators from a range of domestic and international CROs to establish a foundation for suitability and standardization. Furthermore, we supported outreach by introducing the K‑BDS framework and indicators to domestic researchers, providing consultation, training, and promotional activities.

39

ChEMBL

CHEMBL4176932

TRL3 (선도)

Translocator protein (TSPO)

p30536

Alzheimer's disease

D000544

MeSH

Ro5-4864

Discovery of thienopyrrolotriazine derivatives to protect mitochondrial function against Aβ-induced neurotoxicity.

2025-11-28

Toxicity

Cytotoxicity

To a clear 96-well plate (FALCON), the cultured HT-22 cells were seeded in a number of 5,000 per well 24 h prior to the assay. And 25 µL of each test compound (5 µM) was added to each wells. After 24 h of incubation at 37 °C, the cells were treated with 10 µL of MTT solution (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma-Aldrich), and incubated additionally for 2 h at 37 °C. Then, the cells were treated with 135 µL of MTT solubilizing solution (10% Triton-X 100 in isopropanol with 0.1 M HCl), and incubated additionally for 2 h at 37 °C. The % values of cell viability were determined by using the optical density (OD) values of absorbance at 560 nm, and normalized by taking the vehicle control (5 µM of DMSO) as 0%.

HT-22

single concentration

Activity

=

%

88

보고되지 않음

0

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