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This project aims to develop a multiplex molecular diagnostic (Multiplex PCR) kit for the accurate identification of five species (Adenophora triphylla, Adenophora stricta, Codonopsis lanceolata, Glehnia littoralis, and Adenophora remotiflora) used as medicinal herbs. The dataset includes synthetic construct sequences of artificial plasmids, where species-specific SNP target gene regions (rrn23, rpl2, petB, psaA, rpoB) were amplified and inserted into pTOP vectors. These plasmids serve as positive controls for the diagnostic kit and can be utilized as standard reference materials for authenticating the origin and ensuring the quality of medicinal herbs.