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This project aims to develop a multiplex molecular diagnostic (Multiplex PCR) kit for the accurate identification of three species (Schisandra chinensis, Schisandra repanda, and Kadsura japonica) used as medicinal herbs. The dataset includes synthetic construct sequences of artificial plasmids, where species-specific SNP target gene regions (rbcL, rpoC2, matK) were amplified and inserted into pTOP vectors. These plasmids serve as positive controls for the diagnostic kit and can be utilized as standard reference materials for authenticating the origin and ensuring the quality of medicinal herbs.