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This project presents a comprehensive SNP database developed via a standardized and reproducible genetic variant calling workflow. By processing raw NGS reads through a modular pipeline-incorporating BWA, Samtools, Picard, and GATK-we ensure high-fidelity variant discovery. A key feature of this project is the construction of a pseudo-database of SNPs (pseudoDB for short) to enhance the accuracy of variant discovery. The repository offers three distinct data outputs: a pseudoDB, a dbSNP-guided VCF, and a pseudoDB-guided VCF. For species lacking an established dbSNP, the pipeline will automatically construct two distinct resources: a pseudoDB and a pseudoDB-guided VCF. Specifically, the pseudoDB is a VCF generated in the absence (or lack) of a known SNP database, serving as an essential reference alternative for species lacking established resources. This dataset is designed to serve as a reference resource for genomic analysis across diverse species, facilitating genetic diversity studies and molecular breeding. All scripts and configuration files used for this database are publicly available to ensure full computational reproducibility.