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The genome of Escherichia coli W strain (ATCC 9637) was completely sequenced using a hybrid method of Roche/454 pyrosequencing and Sanger sequencing. The Roche/454 pyrosequencing reads, produced at approximately 27.7x coverage, were assembled using gsAssembler to generate about 200 contigs. These were then converted into virtual reads and assembled with fosmid end reads (Sanger) to form scaffolds. The gap regions were filled by end sequencing of PCR products, primer walking of templates (PCR products or fosmid clones), and detailed reassembly work, particularly for two somewhat challenging two prophage copies. The purpose of this BioProject is to provide all Sanger reads and the final assembly generated using the Phred/Phrap/Consed pipeline. Roche/454 pyrosequencing raw data files (SFF) were separately submitted to the KRA database (KAP240861). The completed genome sequence was polished using Illumina GAII and submitted to GenBank, so it may differ from the final results provided in this BioProject.