유전체
Escherichia coli E_coli_HST04_recB_ko, complete genome
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AccessionKAAAAKI01
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Submission date2026-09-29
Project Detail
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BioProject
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KAP242570 |
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프로젝트의 영문 제목
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Acquisition of a Chassis Strain for the Development of a Cell-Free System Enabling Highly Efficient Gene Expression and Protein Production from Linear DNA Templates
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프로젝트의 영문 설명
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Through this study, an E. coli HST04 strain with a knockout of the RecB gene was obtained. This strain is originally deficient in genes involved in genomic DNA methylation, thereby reducing the activity of the restriction and modification system against foreign DNA. In addition, the RecB gene, which is involved in nucleic acid degradation, was further deleted to further eliminate factors contributing to DNA degradation within the intracellular environment of this strain. This strain was utilized for the development of a crude-extract-based cell-free system, and its ability to suppress the degradation of linear DNA was confirmed. Linear DNA is highly susceptible to nuclease-mediated degradation in conventional cell-free systems.
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등록자의 영문 이름
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Yim Sung Sun
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BioSample
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BioSample Accession ID
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KAS24222918 |
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BioSample name
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E_coli_HST04_recB_ko
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Organism
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Escherichia coli
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NCBItaxonomyID
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562
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Strain
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Escherichia_coli_str_K12_substr_MG1655
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Breed
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Cultivar
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Isolate
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Not applicable
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Ecotype
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Host
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Not applicable
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Lab Host
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Isolation Source
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Sample Division
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Bacteria
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DataSet
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Name of submitter's organization
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Korea Advanced Institute of Science & Technology
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Department of submitter
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Graduate School of Engineering Biology
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Country of submitter
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대한민국
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Street address
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291 Daehak-ro
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City
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Yuseong-gu
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State / Province / Region
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Daejeon
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Zip Code (Postal Code)
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34141
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Contact person information
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Publication status
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Unpublished
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Publication
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Reference title
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A chassis strain for the development of a cell-free system enabling highly efficient gene expression from linear DNA templates
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Confirm update of existing submission
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No
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Existing KNA Accession ID
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Sequence Authors
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Sample processing protocol
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Data processing protocol
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Supplementary information
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Release Protocol?
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No
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Confirm full genome
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Yes (even for draft genomes or if a prokaryotic genome assembly may not incude plasmids)
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Description for subset of the genome
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Assembly state
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Each chromosome is in a single sequence and there are no extra sequences
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Assembly level
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Complete Genome
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Confirm de novo assembly
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Yes
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Reference assembly accession ID
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Confirm final version
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Yes
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Locus Tag Prefix
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Reference assembly accession
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Did you submit KRA data for this Genome submission?
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No
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KRA Accession ID
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Assembly date
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2026-07-10
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Sequencing platform - KRA Run
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Sequencing platform
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Sequencing platform
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Assembly method
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Haplotype phasing method
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Assembly name
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hasN
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No
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Did you randomly merge the sequences into a single sequence?
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Do any of the N's represents gaps of completely unknown size gaps?
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Appropriate minimum number of Ns in a row (0-10) that represents a gap
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Representataion of Unknown Gaps
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What type of evidence was used to assert linkage across the assembly gaps?
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Annotation file format
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GFF
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Total length
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4542356
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Total contigs
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1
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Scaffolds or chromosomes Total length
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4542356
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Scaffolds or chromosomes Total contigs
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1
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Sequences belong to?
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Chromosome
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Chromosome
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Organelle
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Plasmid
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Nucleotide Accession
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| File Name | Size | Type | Published | Download | |
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| annot.gff | 2,480,424 2,480,424 | gff | 2026-09-29 | ||
| HST04_recB_ko_bc93_autocycler.fasta | 4,542,360 4,542,360 | fasta | 2026-09-29 |
Contigs
Showing 1-1 of 1
| Accession | Title | Sequence Id | GC% | Length |
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| KAAAAKI010000001 |
Escherichia coli E_coli_HST04_recB_ko, complete chromosome 1
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1
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50.78% | 4,542,356 bp |